Electron Microscopic Studies on Invasion of Malignant Cells to Smooth Muscle Cells / 昆明医科大学学报

The invasion of malignant cells into smooth muscle cells was shown by EM.We dicsovered that the malignant cells adhered to and penetrated the smooth muscle cells with their microvilli and pseudopodia.The microfilament in the microvilli and pseudopodia is the motion skeleton of cancer cells.Studying the structure and function of the microfilament fur- ther is important for researching the metastasis of tumor.

Electron Microscopic Studies on Invasion of Malignant Cells to Collagen / 昆明医科大学学报

The degradation of collagen in the extracellular matrix induced by malignant cells is a very impotant link in the cancerometastasis chain.The invasive and destructive effect of vari- ous types of malignant cells cultured in vitro on the three dimensional gel of the native collagen fibrils was observed by electron microscopy.The cytobiological properties of malig- nant cells were discussed and compared with the normal ones.

Reconstitution of Arterial Wall Using Cell Culture Technique / 昆明医科大学学报

Using cell culture technique, in vitro an artificial arterial wall has been constructed by adding cultured endothelial cells derived from the human umbilical vein to the smooth muscle cells isolated from the human umbilical artery. On the condition of vit C and the endothelial cell growth factor in the medium, both the endothelial cells and smooth muscle cells grew very well and formed living arterial walls with the ability of generation and metabolism. A confluent monolayer of flat endothelial cells and multilayer of smooth muscle cells were observed under the light and electron microscope. Among these cells were plenty of collagenous fiber, elastic fiber and glycoproteins produced by the smooth muscle cells. This blood vessel wall model can be used for studying the physiological and pathological process of arteries.