ABSTRACT
ObjectiveTo construct recombinant adenovirus vector containing human pancreatic and duodenal homeobox factor 1 (PDX1) and detect its expression in human umblical cord mesenchymal stem cells (HUCMSCs). MethodsPDX1 obtained by BgⅢ/XhoI enzyme digestion from pUC57-PDX1 was ligated into the recombinant shuttle vector pShuttle-GFP-CMV to obtain the recombinant shuttle plasmid pShuttle-GFP-CMVPDX1. pShuttle-GFP-CMV- PDX1 was shifted to pAdxsi vector to obtain pAdxsi-GFP-PDX1 virus plasmid. The recombinant plasmid was packaged and amplified in 293 cells. The expression of PDX1 gene and protein in HUCMSCs was detected by fluorescence microscopy, RT-PCB, immunofluorescence, immunohistochemistry, and Western Blot. ResultsPDX1 gene was inserted correctly into shuttle plasmid and the recombinant adenovirus vector was successfully constructed according to the results of sequence and enzyme digestion identification. The adenovirus was effectively transfected into HUCMSCs. RT-PCR verified that PDX1 mRNA was positively expressed in HUCMSCs. Expression of PDX1 protein in the nuclear of HUCMSCs was found by immunofluorescence assay, immunohistochemistry and Western Blot. ConclusionThe adenovirus vector containing PDX1 gene is successfully constructed and effectively expressed in HUCMSCs.
ABSTRACT
Objective To study the relationship in displaying nasopharyngeal carcinoma(NPC)lesions with different MR imagingprotocols.Methods 67cases of NPC proved by pathology were reviewed. Each patient was scanned with six MR imaging protocols (Tra T_1WI, TraT_2WI , SagT_1WI , CorFSIR , TraCE-T_1WI , CorCE-T_1WI ).Results All cases were displayed as mucosal thickening and /or soft tissue masses of nasopharynx. The involved parts were as follows: parapharyngeal spaces in 49 cases(73.1%) ,carotid sheaths in 33 cases ( 49.3% ) , prevertebral muscles in 32 cases ( 47.8% ) , medial pterygoids in 15 cases ( 22.4% ) , lateral pterygoids in 7 cases ( 10.4% ) , pterygoid plates in 9 cases ( 13.4% ) ,pterygopalatine fossae in 5 cases (7.5%), sphenoidal sinuses in 16 cases(23.9%), ethmoidal sinuses in 6 cases(9.0%), maxillary sinuses in 3 cases(4.5%),orbital cavity in 1 case (1.5% ), sphenoid bones in 12 cases(17.9%), petrous apices in 19 cases(28.4%), clivuses in 41 cases(61.2%), cavernous sinuses in 7 cases(10.4%), temporal lobes in 3 cases(4.5%) and cervical lymphnode mestases in 45 cases(67.2%). The lesions displayed by combination of TraT_2WI, SagT_1WI , CorFSIR and CorCE-T_1WI were corresponded with those displayed by the all six MR imaging protocols . Conclusion One or more MR imaging protocols can be optimized for displaying each lesion of NPC. The combination of Tra T_2WI , Sag T_1WI , CorFS IR and Cor CE -T_1WI can display NPC lesions completely.