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Objective To describe the situation of early stage of pelvic floor function and investigate the effect factors in postpartum women.Methods A retrospective survey was conducted,and women who did regular examination and gave birth in Peking University People's Hospital and had an annual pelvic floor examination at 6-12 weeks after delivery from Sep.2012 to Dec.2017 were interviewed.General information and pelvic floor electrical physiological indexes were collected and analyzed.Results Totally 5 143 puerpera were included in the study.The normal strength of type Ⅰ muscle was 52.40％ (2 695/5 143) and the normal strength of type Ⅱ muscle was 52.69％ (2 710/5 143).The abnormal degree of fatigue of type Ⅰ muscle was 55.84％ (2 872/5 143) and the abnormal degree of fatigue of type Ⅱ muscle was 27.34％ (1 406 / 5 143).Pelvic floor dynamic pressure was (93 + 37) cmH2O (1 cmH2O=0.098 kPa).The incidence of stress urinary incontinence (SUI) was 23.49％ (1 208/5 143) after delivery and it was as high as 57.58％ (95/165) for women who had family history of pelvic floor dysfunction.Family history of pelvic floor dysfunction was the risk factor of the fatigue index of type Ⅰ and type Ⅱ muscle (P＜0.05).The incidence of SUI was associated with vaginal delivery and age (＞30 years old) and family history of pelvic floor dysfunction (P＜0.01).Conclusions Pelvic floor electrical physiological indicators are reduced in about half of women in early postpartum stage.Age (＞30 years old) and vaginal delivery and family history of pelvic floor dysfunction are the risk factors.
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Objective] To investigate and compare with western medicine on the advantages of traditional Chinese medicine in the treatment of subacute thyroiditis. [Method] Select subacute thyroiditis clear diagnosis, single disease cases in clinical work.For patient treated with pure Chinese medicine internal and external treatment. Observation, comparison and analysis of the degree of symptom relief, the size of the side effect, the prognosis of patients and the satisfaction of patients with medical treatment.Discussion on Chinese medicine in the treatment of subacute thyroiditis the high compliance of advantages, the advantage of fewer side effects,therapeutic advantage and the advantage of improving the prognosis and reducing the sequela of disease. [Result] Through clinical observation and comparison, traditional Chinese medicine to western medicine in the treatment of subacute thyroiditis, good treatment compliance, curative effect are exact, side effects rarely, high patient satisfaction,good prognosis show obvious advantage. [Conclusion] Chinese medicine treatment of subacute thyroiditis, compared with the western medicine has obvious advantage for early clinical intervention, controling an illness as soon as possible, improving prognosis, reducing hypothyroidism incidence are of great significance.
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Objective To investigate the relationship between apparent diffusion coefficient (ADC)value of rectal adenocarcinoma on DWI and its pathological grading.Methods The ADC values of 46 rectal adenocarcinomas were measured and compared with their histopathological grades.Results The 46 rectal adenocarcinomas included well differentiated adenocarcinomas in 14,moderate-ly differentiated ones in 20,and poorly differentiated ones in 12.The ADC values of well,moderately and poorly differentiated ade-nocarcinomas were (1.125±0.103)×10 -3 mm2/s,(1.030±0.098)×10 -3 mm2/s and (0.922±0.091)×10 -3 mm2/s,respective-ly,exhibiting a statistical difference (χ2 =1 7.35 1,P =0.000).Mann-Whitney U test showed that difference in ADC value between different histopathological grades was statistically significant.Conclusion ADC value of rectal adenocarcinoma can be used as a bio-marker for cell grading to guide treatment decision and prognosis assessment.
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Cathepsin G (CG) is one of the serine proteases of neutrophils,and its mRNA is specifically increased in the promyelomonocytic stage of neutrophils and abnormal in several acute myeloid leukemias (AML).Meanwhile,CG is one of AML-associated antigens with strong immunocompetence,it is an important target of immunotherapy in AML.In this paper,the role of CG in the diagnosis and immunotherapy of AML were reviewed.
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Objective To observe the level of dopamine and its receptor in striatum from conditioned place preference (CPP) of rats administrated of morphine.And explore the mechanism of opioid-psychic dependence involving the levels of neurotransmitter and receptor.Methods CPP model was validated in morphine-dependence rats for 10 days.Striatum samples were harvested from two separate groups involving the saline group and morphine treated group (n =10 per experiment).The DA contents in striatum were detected in rats with colorimetry.Immunohistochemistry was applied to detect the differential expression levels of dopamine receptor 2 (DRD2)in samples.Results Compared with the saline group,higher content of neurotransmitter dopamine was observed in the morphine dependent rats (7.63 ±0.98 vs 5.23 ± 1.01,P＜0.01),while the expression level of DRD2 was down regulated (0.06 ± 0.02 vs 0.08 ± 0.02,P ＜ 0.01).Conclusion The increased expression of neurotransmitter dopamine and the decreased of DRD2 may be contributed to morphine-induced psychological dependence in morphine dependent rats.
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Objective To detect CK-19 mRNA expression by quantitative real-time RT-PCR in axillary drainage fluid of rectal cancer and investigate its clinical significance.Methods Axillary drainage fluids were collected from 59 patients with rectal cancer and 15 patients with benign abdominal lesion from Sep.2010 to Dec.2010.Level of CK-19 mRNA in axillary drainage fluid was detected using specific primers by real-time RTPCR.The data were statistically analyzed to investigate the relationships between CK-19 mRNA level and tumor invasion,lymph node status,tumor stage and tumor differentiation level.Results The positive rate of CK-19 mRNA expression in patients with rectal cancer was 67.8％,which was significantly higher than that in patients with benign abdominal lesion.The expression of CK-19 mRNA was significantly correlated with the depth of tumor invasion,lymphnode status,tumor stage and histopathological differentiation( P ＜ 0.05 or P ＜ 0.01 ).Ck129 mRNA expression was associated with the pathological level,the higher of the lymph node translation level,the higher expression in the axillary drainage fluid after rectal cancer surgery (r =0.674,P =0.021 ).The lower of the lymph node differentiation level,the higher expression in the axillary drainage fluid after rectal cancer surgery (r =-0.741,P =0.014).Conclusion Quantitative detection of CK-19 mRNA in axillary drainage fluid of rectal cancer by RT-PCR could enhance the diagnostic sensibility of colorectal cancer micrometastases.RT-PCR assay is suitable for predicting peritoneal micrometastasis of rectal cancer,which is a reference for postoperative treatment and prognosis prediction.
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Justified the necessity of remote pathology consultation in China, and described the basic approach of such consultation in terms of the conditions, organizational framework, specialists and consultation process of the hospital. on the basis of benefit in pationts, the consultation helps the development of the pathology department and other specialist departments at the same time, builds initially a pathology quality control system, and accelerates the multi-discipline diagnosis and treatment approach. Expect to encourage contemplation on international remote pathology consultation in an effort to improve such a practice for the benefit of patients.
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In this study, we collected human umbilical cord blood and separated the autologous serum and clear cells. Then the human mesenchymal stem cells were cultured with the use of autologous serum and fetal serum, respectively. By identifing surface markers with flow cytometry and testing the osteogenic, adipogenic and cardiomyocytes differentiation capacity of the cells, we affirmed that the cultured cells are mesenchymal stem cells. Among 21 cases of umbilical cord blood, 11 cases using fetal bovine serum and 15 cases using autologous successfully cultured mesenchymal stem cells from umbilical cord blood. The UCB-MSCs did not express CD34 and CD45, but they expressed CD29, CD44 and CD105, and they were successfully induced into osteogenic cells, adipogenic cells and cardiomyocytic-like cells. All of these suggest that using autologous serum to culture UCB-MSCs is better than using fetal bovine serum, and the autologous serum cultured UCB-MSCs can also be induced into osteogenic cells, adipogenic cells cardiomycyote-like cells.
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Humans , Cell Differentiation , Physiology , Cell Division , Cells, Cultured , Culture Media , Fetal Blood , Cell Biology , Mesenchymal Stem Cells , Cell Biology , SerumABSTRACT
AIM: To observe the clinical efficacy of Dengzhan Shengmai Capsules(Herba Erigerontis,Radix et Rhizoma Ginseng,Radix Ophiopogouis,Fructus Schisandrae chinensis) in improving type 2 diabetes insulin resistance. METHODS: 98 patients were randomly divided into two groups: the control group(50 cases) were treated with conventional therapy,the treatment group(48 cases) were treated with Dengzhan Shengmai Capsules based on conventional therapy.These two groups were all treated for 2 months.FPG,FINS,2hPG,HbA_1C and ISI were observed before and after treatment. RESULTS: The total effects of the treatment group(91.67%) was higher than that of the control group(40.00%)(P