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Objective@#To explore the clinical value of cystatin C in predicting the severity of community-acquired pneumonia(CAP).@*Methods@#From January 2016 to August 2016, the clinical data of 122 patients with CAP in Shengjing Hospital of China Medical University were retrospectively analyzed.The patients were divided into low-risk group(74 cases) and middle-high risk group(48 cases) according to pneumonia severity index (PSI). The cystatin C, procalcitonin, C-reactive protein(CRP), white blood cell (WBC) count, neutrophil percentage(NE%), D-dimer and fibrinogen(FIB) levels in hospitalized patients were detected.The relationship between cystatin C and the above related indicators and the PSI was evaluated.Draw ROC curve with cystatin C and above indicators to predict severity of CAP.@*Results@#In the low-risk group, the levels of procalcitonin, CRP, WBC count, NE%, D-dimer, FIB were[0.08(0.04, 0.23)]ng/L, [42.35(12.52, 93.70)]mg/L, [7.85(6.23, 10.38)]×109/L, (66.87±13.49)%, [215.00(134.25, 410.25)]μg/L, [4.52(3.71, 5.14)]g/L, respectively, which in the middle and high risk group were [0.25(0.07, 0.54)]ng/L, [74.30(40.45, 122.75)]mg/L, [7.90(5.78, 10.63)]×109/L, (73.97±10.77)%, [417.50(239.75, 730.00)]μg/L, [4.57(3.87, 5.08)]g/L, respectively, the differences of NE%, procalcitonin, CRP, D-dimer between the two groups were statistically significant(t=3.064, U=3.024, 2.800, 3.771, all P<0.05), the WBC count, FIB had no statistically significant differences between the two groups (all P>0.05). In the low-risk group, the level of cystatin C was[0.82(0.68, 0.91)]mmol/L, which in the middle-high risk group was[1.32(1.12, 1.54)]mmol/L, the difference between the two groups was statistically significant(U=7.978, P<0.001). When the level of cystatin C was 0.975 mmol/L, the sensitivity for the diagnosis of pneumonia severity was 95.8%, with a specificity of 85.1%.@*Conclusion@#Monitoring cystatin C levels can be used as an indicator of inflammatory response and can predict the severity of pneumonia.
ABSTRACT
Objective To explore the clinical value of cystatin C in predicting the severity of community-acquired pneumonia(CAP).Methods From January 2016 to August 2016,the clinical data of 122 patients with CAP in Shengjing Hospital of China Medical University were retrospectively analyzed.The patients were divided into low-risk group(74 cases) and middle-high risk group (48 cases) according to pneumonia severity index (PSI).The cystatin C,procalcitonin,C-reactive protein (CRP),white blood cell (WBC) count,neutrophil percentage (NE％),D-dimer and fibrinogen(FIB) levels in hospitalized patients were detected.The relationship between cystatin C and the above related indicators and the PSI was evaluated.Draw ROC curve with cystatin C and above indicators to predict severity of CAP.Results In the low-risk group,the levels of procalcitonin,CRP,WBC count,NE％,D-dimer,FIB were[0.08 (0.04,0.23)] ng/L,[42.35(12.52,93.70)] mg/L,[7.85 (6.23,10.38)] x 109/L,(66.87 ±13.49) ％,[215.00 (134.25,410.25)] μg/L,[4.52 (3.71,5.14)] g/L,respectively,which in the middle and high risk group were [0.25 (0.07,0.54)] ng/L,[74.30 (40.45,122.75)] mg/L,[7.90 (5.78,10.63)] × 109/L,(73.97 ± 10.77) ％,[417.50 (239.75,730.00)] μg/L,[4.57 (3.87,5.08)] g/L,respectively,the differences of NE％,procalcitonin,CRP,D-dimer between the two groups were statistically significant (t =3.064,U =3.024,2.800,3.771,all P ＜0.05),the WBC count,FIB had no statistically significant differences between the two groups (all P ＞ 0.05).In the low-risk group,the level of cystatin C was [0.82 (0.68,0.91)] mmol/L,which in the middle -high risk group was [1.32 (1.12,1.54)] mmol/L,the difference between the two groups was statistically significant (U =7.978,P ＜ 0.001).When the level of cystatin C was 0.975 mmol/L,the sensitivity for the diagnosis of pneumonia severity was 95.8％,with a specificity of 85.1％.Conclusion Monitoring cystatin C levels can be used as an indicator of inflammatory response and can predict the severity of pneumonia.
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Objective To observe HA14-1 sensitizes Lewis lung carcinoma in mice to cyclophosphamide (CTX) and to explore its possible mechanism. Methods Forty Lewis lung carcinoma model mice were randomly divided into 4 groups:normal saline group,CTX group, HA14-1 group,CTX+HA14-1 group. After the treatment of 7 days,all of the mice were killed on the 22nd day of tumor inoculation. The tumor volume growth curve of each group was described;tumor inhibition rate was caculatued;Bcl-2,Bax,Caspase-9 protein expression levels before and after the treatment were determined by immunohistnehemistry. Results Compared to the normal saline group,HA14-1 group had no significant effect on inhibiting tumor volume,and the tumor volume in HA14-1 group increased less slowly than that of CTX group, HA14-1 group and CTX+HA14-1 group. Compared to the normal saline group, the tumor inhibition rate of HA14-1 group had no significant increase (P> 0.05),while that of CTX group and CTX + HA14-1 group increased significantly (P< 0.05);compared to the CTX group,the tumor inhibition rate of CTX + HA14-1 group increased significantly (P < 0.05). Bcl-2 protein expression levels in CTX group,HA14-1 group and CTX+HA14-1 group were lower than that in the normal saline groups compared to CTX group,Bcl-2 protein expression of CTX + HA14-1 group reduced significantly. Compared to the normal saline group,the expression levels of Bax and Caspase-9 protein in CTX group, HA14-1 group and CTX+HA14-1 group increased significantly (P< 0.05);compared to CTX group,CTX + HA14-1 group increased more significantly (P < 0.05). Conclusion HA14-1 might enhance the efficiency of CTX chemotherapy via inhibiting the expression of Bcl-2, increasing the expression of Bax and caspase-9 and promoting tumor cell apoptosis.