ABSTRACT
Objective To investigate the expressions and clinical significance of Id1 in diffuse large B‐cell Lymphomas (DL‐BCL) tissue and Id1 gene in bone warrow cell .Methods Forty cases of DLBCL(observation group) and 25 cases of reactive lymph‐oid hyperplasia (control group) were included in this study which were admitted by our hospital from October 2011 to October 2014 .The expression of Id1 proteins in DLBCL and reactive lymphoid hyperplasia were detected by imunohistochemical technique . The expression of Id1 genes in all patients′marrow cells was detected by reverse‐transcriptase polymerase chain reaction .The data were collected and analyzed by designed person .Results In 40 DLBCLs ,the positive rate of Id1 were 75 .00% (30/40) ,which was higher than in RHs 32 .00% (8/25) ,with statistic difference(P= 0 .001) .Id1 protein was not correlated with sex and age(P>0 .05) ,but was correlated with clinical stage ,LDH level and extranodal infiltration(P<0 .05) .The expression of Id1 genes in mar‐row cells in DLBCL was higher than in RH (Id1mRNA level 2 .80 ± 0 .87 vs .1 .37 ± 0 .51 ,P<0 .05) ,and also correlated with clini‐cal stage ,LDH level and extranodal infiltration(P<0 .05) .Conclusion The expression of Id1 proteins and genes are much higher in DLBCL tissue and marrow and probably related to the prognosis of DLBCL .This discovery would contribute to predicting prognosis of DLBCL ,w hich also could be a therapeutic target of DLBCL in the future .
ABSTRACT
Objective To investigate the expression level and clinical significance of T‐cadherin protein and gene in the mixed cell type of hybrid hodgkin lymphoma .Methods The lymph nodetissue samples were obtained from 45 hybrid Hodgkin lymphoma patients and 20 reactive hyperplasia lymphoid patients .The expression level of T‐cadherin of these samples were detected by immu‐nohistochemical staining and RT‐PCR .The relationship of clinic pathological features and prognosis in hybrid Hodgkin lymphoma patients were analyzed by the expression level of T‐cadherin .Results The positive expression rates of T‐cadherin in protein hybrid hodgkin lymphoma and reactive hyperplasia lymphoid tissue specimens were 31 .1% (14/45) and 90 .0% (18/20)(P 0 .05) .Multiva‐riate logistic regression analysis demonstrated TNM stage and T‐cadherin negative expression were independent factors of lymph node involvement .Log‐rank test revealed the two‐year survival rate in hybrid Hodgkin lymphoma patients of T‐cadherin negative and positive expressionwere 83 .9% and 92 .9% (P> 0 .05) ,three‐year survival rate were 71 .0% and 85 .7% (P> 0 .05) ,five‐year survival rate were 41 .9% and 78 .6% (P< 0 .05) .Kaplan‐Meier survival analysis showed the five‐year survival in T‐cadherin nega‐tive expressionwas lowersignificantly than T‐cadherin positive expression (P< 0 .05) .Conclusion T‐cadherin was low expression in hybrid Hodgkin lymphoma and correlated with TNM and lymph node involvement .T‐cadherin may be a new molecular marker to evaluate the prognosis of hybrid hodgkin lymphoma .