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Objective To establish an experimental choroidal neovascularization(CNV)model in brown norway(BN)rats by laser infusion in order to study the evolution of CNV during 3 weeks with intravitreaI injection of 1 μl(25 μg)bevacizumab,and make a preliminary investigation of the treatment effect of bevacizumab on experimental CNV. Methods Four groups of 48 BN rats were photocoagulated by krypton laser in 1 eye to induce CNV model in 1 eye,in which 4 groups received 1 μl(25 μg)bevacizumab intravitreal injection 1 week after photocoagulation and the other groups received the same volume intravitreall injection of balanced salt solution(BSS)as control.Fundus fluorescein angiography(FFA)examinations were taken on 1,2,3 week and the eyes were enucleated and processed for histopathologic examination.The areas of CNV were measured in the early phase of FFA.FⅧ-Rag protein was investigated with immunohistoehemistry and was semiquantkatively analyzed. Results The area and the leakage of CNV in bevacizumab treated BN rat eyes were obviously reduced as compared with the control group at each interval.In the treatment group,the expression of FⅧ-Rag was depressed.The examination of FⅧ-Rag and FFA verified the depression of CNV as compared with the control group at each interval.The density of treatment was lower than of control group(P＜0.01).After bevacizumab treatment for 2 weeks,the area of CNV and the expression of FⅧ-Rag were decreased[(0.920±0.634)mm2 and 35.57±10.52,respectively),as compared with the control group[(2.489±0.590)mm2 and 175.37±25.20]. Conclusions Intravitreal bevacizumab injection can inhibit the development of CNV.
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Objective To study the ecotoxicological effect of Mn,Cd and Mn-Cd combined contamination on crops.Methods Chinese cabbage(Brassica pekimensis),lettuce(Lactuca sativa var.angustana Irish),cowpea(Vigna unguiculata) and alfalfa(Medicago sativa L.) seeds were used in seed germination and root elongation tests.Results Research findings indicated that inhibition rates of heavy metals(individual or combined) on the seed germination were much lower than those on the root elongation.Inhibition rate of root elongation proved positively correlated to the concentration of Mn and Cd(P
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SOD.Conclusion Mn,Cd and Mn-Cd combined exposure has a significant inhabitation on cowpea growth with Cd alone being heaviest and Mn lowest.The physiological indicators show that when combined pollution occurs,an antagonistic effect between Mn and Cd is exhibited.