ABSTRACT
Objective:To explore the feasibility of using multivariate statistical analysis to evaluate the quality of Astmgali radix from different habitats. Methods:The contents of Astragaloside saponinⅠ, Astragalus saponin Ⅱ, Astragaloside Ⅲ and Astragaloside A were determined by UPLC-ELSD. The components of astragalus saponins from different habitats were analyzed by TOPSIS and cluster thermogram.Results:TOPSIS analysis showed that the comprehensive quality of Astmgali radix samples from Shanxi, Gansu and Inner Mongolia was related(were 0.297 3, 0.346 0, 0.322 5), and the whole quality of S13 and S14 from Shanxi and N5 from Inner Mongolia were higher than others. Cluster thermogram showed that Astmgali radix was grouped into three groups according to region, and the quality difference of Astmgali radix was mainly reflected on Astragaloside saponinⅠand Astragalus saponin Ⅱ. Conclusions:The theory of multivariate statistical analysis is perfect, objective and reliable. It can be used as a reference for comprehensive quality evaluation of Traditional Chinese Medicine (TCM), selection of excellent germplasm resources and traceability of origin of TCM.
ABSTRACT
OBJECTIVE:To establish a method for the simultaneous determination of flavonoids components in Astragali Ra-dix,and to explore the relationship among flavonoids components,varieties,origins and planting patterns. METHODS:HPLC was performed on the column of Venusil ASB with mobile phase of acetonitrile-0.3% formic acid (gradient elution) at a flow rate of 1.0 ml/min,detection wavelength was 260 nm,and column temperature was 25 ℃. Medicinal material quality of Astragalus mem-branaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao and A. membranaceus (Fisch.) Bge of wild and cultivated from different province was compared. RESULTS:The linear range of the mass concentration was 0.008 9-2.224 mg/ml for calycosin glucoside (r=0.999 5),0.005 2-1.3 mg/ml for ononin(r=0.999 6),0.002 8-0.697 6 mg/ml for calycosin(r=0.999 9)and 0.002-0.5 mg/ml for formononetin (r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 1%;recoveries were 99.52%-100.74%(RSD=0.41%,n=6)for calycosin glucoside,98.84%-100.60%(RSD=0.60%,n=6)for ononin ,98.47%-101.74%(RSD=1.08%,n=6)for calycosin,100.10%-101.59%(RSD=0.32%,n=6)for formononetin. In terms of varieties,the contents of calycosin glycosides,ononin and flavonoids in A. membranaceus(Fisch.)Bge. var. mongholicus(Bge.)Hsiao were higher than those of A. membranaceus (Fisch.)Bge,but the contents of calycosin and formononetin were less than those of A. membranaceus (Fisch.)Bge;in terms of origins,calycosin glycosides and flavonoids of Inner Mongolia and Shanxi held the highest contents,fol-lowed by those of Northeast China and Gansu,and lowest in Shandong,Anhui and Shaanxi;in terms of planting patterns,the con-tents of calycosin glycosides,ononin and flavonoids of wild Astragali Radix were higher than those of cultivated varieties,and the contents of calycosin and formononetin of cultivated varieties were higher than those of wild ones. CONCLUSIONS:The method is simple,stable and reproducible,and can be used for the simultaneous determination of flavonoids components in Astragali Radix. The flavonoids components show great differences in Astragali Radix from different origins,and they are affected by varieties,ori-gins and planting patterns.