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To analyze the psychological experience of close contacts with COVID-19 during the centralized isolation medical observation (centralized isolation for short) period, 17 close contacts of COVID-19 who received medical isolation observation at designated locations in a city of China were selected by purposive sampling using qualitative study methods, and the interviewees were interviewed by full-coverage structured telephone. By analyzing the obtained data and summarizing the themes, it was found that the psychological status of the COVID-19 close contacts during the centralized isolation period could be classified into three themes: obvious negative emotions, somatization of psychological problems, and demand for social support. During the epidemic, when close contacts were quarantined as high-risk groups, they often had complex and difficult psychological experiences. Relevant departments should actively carry out online guidance channels and offline knowledge dissemination, actively conduct psychological interventions for close contacts, assist them to develop life plans to enrich the life of the centralized isolation period. At the same time, relevant departments should do a good job in logistics and life support services, create a comfortable isolated living environment, and effectively relieve the negative emotions of close contacts of COVID-19 during centralized isolation through a variety of positive and effective measures to ensure physical and mental health.
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Objective:To investigate the effect of irradiation on the expression of miR-150-5p and miR-23a-3p in human peripheral blood serum by collecting peripheral blood of tumor patients before and after radiotherapy, so as to provide scientific basis for finding radiation biomarkers.Methods:A total of 63 tumor patients treated with radiotherapy from October 2021 to March 2022 were enrolled in this study. The relative expression levels of miR-150-5p and miR-23a-3p in peripheral blood serum in these patients were detected using the real-time fluorescence quantitative PCR (qPCR) before and after radiotherapy. The differential changes in the expression levels of the two miRNAs in the peripheral blood serum of the patients before and after radiotherapy were compared, and their relationships with factors such as cancer types were analyzed.Results:The relative expression levels of miR-150-5p and miR-23a-3p in peripheral blood serum of the patients after radiotherapy were significantly lower than those before radiotherapy ( t = 4.97, Z = -2.77, P < 0.05). Among different cancer types, the relative expression level of miR-150-5p in the patients with breast cancer, esophageal cancer, or other digestive tract cancer decreased after radiotherapy ( t = 3.47, 2.47, 2.87, P < 0.05), and the relative expression level of miR-23a-3p in the patients with digestive tract cancer decreased after radiotherapy ( Z = -1.99, P < 0.05). The changes in the expression level of miR-150-5p before and after radiotherapy were not affected by gender, age, chemotherapy, and cancer type ( P > 0.05). By contrast, the changes in the expression level of miR-23a-3p before and after radiotherapy were significantly affected by gender, age, and chemotherapy ( t=2.04, -3.34, -2.29, P<0.05). Conclusions:The expression of miR-150-5p in the serum of tumor patients may be affected by radiotherapy, which has the potential to be used as a biological indicator of radiation.
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Objective:To analyze the adverse reactions, cosmetic effects and the improvement in positioning of breast cancer patients who received breast-conserving operation followed by hypofractionated radiotherapy and conventional radiotherapy based on the positioning of body membrane method with windowing method.Methods:From January 2019 to April 2020, 75 patients with breast cancer were randomly divided into two groups: hypofractionated radiotherapy (38 cases) administered with 42.4 Gy/16 fractions (2.65 Gy/fraction), and conventional group (37 cases) with 50 Gy/25 fractions (2.0 Gy/fraction). All of them were located by thermoplastic film, and the upper parts of the thermoplastic film were cut along the root of the breast. The adverse reactions, cosmetic effect and follow-up survival of the two groups were observed.Results:During the follow-up period, there was no recurrence in the two groups within 8 months. The local control rate and the survival rate of all the patients were both 100%. The total incidence of acute skin reaction in the two groups was 36.84% (14/38) in the hypofractionated group and 67.57% (25/37) in the conventional group ( χ2=7.09, P<0.05). The excellent and good rate of cosmetic effect was 89.47% (34/38) in the hypofractionated group and 78.38% (29/37) in the conventional group, but the difference was not statistically significant ( P>0.05). There were not statistically differences in the late skin reaction, leucopenia and thrombocytopenia between the two groups ( P>0.05). Conclusions:Breast-conserving surgery followed by hypofractionated radiotherapy with the positioning of body membrane windowing localization method is feasible and safe. Also, it can significantly reduce the acute radiation skin reactions. But the long-term effects still need to be confirmed by long-term follow-up.
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Objective To explore the nuclear emergency nursing rescue process, and ensure safety and effectiveness of implementation of nuclear emergency nursing rescue. Methods Based on the analysis of the existing national standards, and on the basis of literature review, the method of Delphi was used to collect 23 experts′suggestions, and to make a preliminary draft nuclear emergency nursing rescue process on site. Results The experts' enthusiasm was very high, and the degree of authority (Cr) coefficient was 0.91. A on-site rescue unit partition graph, and two rescue unit process that were preliminary sorting and decontamination, and an on-site rescue process had been drew up. Conclusions After two rounds of expert enquiry, the emergency nursing rescue process is reasonable. And the study could provide objective basis for the implementation of the nuclear emergency nursing rescue.
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A stimulate method for determination of polybrominated diphenyl ethers ( PBDEs) and derivatives (OH-PBDEs and MeO-PBDEs), tetrabromobisphenol A (TBBPA), hexabromocyclododecane (HBCD) in egg samples was developed by gel permeation chromatography ( GPC) and dispersive solid phase extraction ( DSPE) combined with liquid chromatography tandem mass spectrometric ( HPLC-MS/MS) and gas chroma-tography-negative chemical ionization mass spectrometry ( GC-NCI/MS ) . The analytes were extracted with mixture of hexane and dichloromethane (1∶1, V/V) by accelerated solvent extraction (ASE), and purified by 100 mg C18 dispersive solid phase extraction ( SPE) sorbents followed with gel permeation chromatography (GPC) , and then analyzed by liquid chromatography tandem mass spectrometric (HPLC-MS/MS) and gas chromatography-negative chemical ionization mass spectrometry (GC-NCI/MS), respectively. The quantita-tion was carried out external standard method. The recoveries of objects were 64. 5%-97. 2% and 65. 6%-109 . 2% ( except BDE85 was 54 . 8%, OH-BDE-137 was 47 . 4%) spiked at 1 . 0 μg/kg or 5 . 0 μg/kg in egg white and egg yolk, respectively. The relative standard deviations (RSDs) were less than 20. 2%. The limits of quantitation (LOQ) for the object were 0. 01-0. 2 μg/kg.
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Objective To investigat the safe time of SVC interruption in New Zealand rabbits, and help to find the safe time ofSVC interruption in chnical operation. Methods 25 New-Zealand rabbits were separated into 3 groups randomly. Bi-SVC was cross-clamped for one hour, two hours in animal models. The femoral artery or SVC pressures were monitored. The control group (sham op-eration group, 5) were opened chest both sides and monitored femoral artery pressure and SVC pressure for 2 hours. Samples of cere-brum, cerebellum and medulla of rabbits immediately post-operation were studied for water content, light and electric microscopies.The tiny change of brain cells in brain mantle, hippocampus and thalamencephal were observed under electron microscope. Results(1) The SVC pressure of rabbits varied regularly in occlusion groups, which displayed as "upgrade-degression- upgrade again-retain".(2) There was no obvious difference in water content of brain between the one hour group and the control group, both of which weremuch lower than two hours group. (3) The brain tissues were almost in normal construction in one hour group, but encephaledema wasobserved in two hours group. The pathological change of cony brain cells was light and reversible in one hour group. However, in twohours group, necrosis of neuron ceils in brain could be observed, which was irreversible. Concerto The animal model of acuteSVC obstruction was built successfully. Interruption of the SVC for 1 hour was proved safe, with longer interruption of SVC 2 hours,obvious encephaledema and necrosis of neuron cells can be observed.
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Object: To know the situation of college students' knowledge about STDs and to help special department to give an aimed education. Methods: 1020 students were investigated by questionnaire and the data analyzed by the Epi5.0. Result: College students' knowledge about STDs were very little. Just 12.2% of these students know exactly the name of STDs which should be key prevent. About the knowledge of transmission and prevention of STDs, the married, older, higher educated and medical students were better than the corresponding other students. Conclusion: The education of knowledge about STDs should be strengthened in university, especially for freshmen.
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Objective: To know the situation of college students to grasp knowledge about generation health in Xi'an and to help the department to give an aimed education. Methods: 1020 students were investigated by questionnaire and the data be analyzed by the software Epi5.0. Result: College students' knowledge about generation health and STDs were very little. About 1/3 of them didn't know how to make urgent contraception, however the proportion of abortion were so high in university. Conclusion: The education of knowledge about generation health and guidance about contraception should be strengthened in university, especially in freshmen.
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Objective: To understand AIDS knowledge of the college students,to provide scientific evidence for making out the strategy of health education and synthetic prevention measure purposely.Methods An anonymous questionnaire was conducted with face to face interview to investigate the college students in Xi'an, and the software Epi info 5.0 was used to perform the statistical analysis of the data. Results 70.5% of university students knew the whole name of AIDS. As to three ways of HIV transmission, the investigation shows that 49.1% of college students knew three main ways. The 26.5% of college students mistakenly think that mosquito could transmit HIV. In general, the old, the married the literate, and medical students get more knowledge about AIDS than the young,the unmarried,the illiterate, and other major students. Conclusion The college students who are one of the most dangerous groups infected AIDS have poor knowledge. The situation of poor AIDS knowledge is extremely unfit to the prevalent tendency of AIDS, which our country is being faced. We suggest that the college students should be educated intensively on the health knowledge of AIDS especiallis beingy for freshmen.