ABSTRACT
Objective To explore the role of the sensory neuropeptide in the chronic cough induced by postnasal drip syndrome(PNDs).Methods Patients of PNDs with and without chronic cough who came to the First Affiliated Hospital of Ji Nan University between Mar.2004 and Sep.2005 were enrolled and induced sputum by hypertonic saline aerosol inhalation was performed.Twenty-three cases of PNDs with chronic cough and 16 cases of PNDs without chronic cough were collected.Substance P(SP)and calcitonin gene-related peptide(CGRP)in the supernatant of the induced sputum were measured by radioimmunoassay.Results SP and CGRP in the supernatant of the induced sputum were significantly higher in the patients with chronic cough induced by PNDs[SP(345.14?72.58)mg/L,CGRP(573.78?210.96)mg/L]as compared with those normal subjects[SP(168.14?56.97)mg/L,CGRP(227.69?70.84)mg/L,P
ABSTRACT
AIM:To investigate the role of nerve growth factor (NGF) and leukemia inhibitory factor (LIF) in the expression of IL-4 and IL-5 in the splenic lymphocytes of asthmatic rats. METHODS:Sixteen Sprague-Dawley rats were randomly divided into two groups:control group (n = 8) and asthma group (n = 8). The asthmatic model was set up by intraperitoneal injection of 100 mg ovalbumin and 100 mg aluminum hydroxide,and receiving nebulization aspiration of 1% ovalbumin solution 2 weeks later. The splenic lymphocytes from the control rats and asthmatic rats were isolated and cultured with exogenous NGF or LIF at different doses. The mRNA expression of IL-4 and IL-5 in the splenic lymphocytes was respectively detected by reverse transcription-polymerase chain reaction (RT-PCR). The secretions of IL-4 and IL-5 were respectively detected by ELISA. RESULTS:Compared to control group,the expression of IL-4 and IL -5 (P