Lipoleiomyoma of the left broad ligament with dermoid cyst in ipsilateral ovary and synchronous multiple benign lesions of female genital tract: An unusual association.

Lipoleiomyoma of the uterus is a rare variant of leiomyoma, and lipoleiomyoma of the broad ligament is still rarer, with only a handful of cases being reported. The present case was a perimenopausal woman who presented with a huge lower abdominal mass. Ultrasonography and computed tomography showed a heterogeneous solid mass in the left adnexa. The histopathological findings confirmed the nature of the lesions as a benign lipoleiomyoma with dermoid cyst of the left ovary and its other associated benign lesions, were the interesting features seen in this case which were not suspected clinically and radiologically.

Facial and Palatal Talon Cusp on a Mesiodens Associated with Hyperdontia- A Rare Occurrence.

Supernumerary teeth or hyperdontia are those that are additional to the normal complement of teeth. Mesiodens is an extra tooth most commonly seen in the premaxillary region, while talon cusp is a well demarcated additional cusp extending form cingulum or cemento- enamel junction to the incisal edge. It is usually present on labial or palatal surface of primary and permanent anterior teeth. Occurrence of talon cusp on a mesiodens is an uncommon phenomenon. This paper presents a rare case of facial and palatal talon cusp on a mesiodens in association with hyperdontia, reported in a 12-year old girl.

Isolation and Physico-Chemical Characterization of Extracellular Ligno-Cellulolytic Enzymes of Pleurotus Pulmonarius in Submerged Fermentation.

Pleurotus pulmonarius, a member of oyster mushroom can produced lignocellulosic enzymes laccase, peroxidise and cellulase in liquid potato-dextrose medium in submerged stationary condition. The lignocellulolytic activities were assayed using the extracellular culture filtrate which was partially purified using 0- 80% ammonium sulphate saturation. Different physico-chemical studies were performed using the partially purified culture filtrate. The fungus produced more laccase and peroxidase than the cellulase. The optimum laccase production was found on 17th day whereas cellulase & peroxidase productions were found on 9th& 10th day, respectively. Km of laccase is 4.1mM against guaiacol and 1.25 mM against o-dianisidine whereas Km of peroxidase was 0.72mM and cellulase was 0.06 mM. Optimum pH of laccase was 6.0 but for peroxidase and cellulase it was 7.0. The temperature optima of cellulase (50⁰C) was more than laccase (40⁰C) and peroxidase (30⁰C). All the lignocellulosic enzymes showed a wide range of temperature and pH stabilities. Laccase and peroxidase were fully inhibited by NaCl but it was not so effective against cellulase. P. pulmonarius showed higher ligninolytic (Laccase and peroxidase) activity than cellulolytic (cellulase) activity. The lignocellulosic enzymes isolated from submerged fermentation of P. pulmonarius might be industrially significant as they showed a wide range of temperature and pH stabilities.