ABSTRACT
Resistance to higher antimicrobial agent is commonly seen in gram negative bacilli. This issue is a challenging problem to the medical practitioners in addition to it is financial impact on the health care system. To document the prevalence of multi drug resistant gram negative bacilli isolated from urine of patients attending the Urology Department of Tertiary care Hospital of western India in year 2008. Out of total 328 isolates, 118 [35.98%] E.coli, 72 [21.95%] Klebsiella, 64 [19.51%] Pseudomonas aeruginosa, 30 [9.15%] Acinetobacter, 18 [5.49%] Proteus vulgaris, 18 [5.49%] Proteus mirabilis, 6 [1.83%] Providencia rettgerii, 2 [0.61%] Citrobacter freundii. Out of these isolates, 228 [69.51%] were b-lactamase positive, while 100 [30.51%] were beta-lactamase negative. Out of 228 beta-lactamase positive, 104 [45.61%] were AmpC beta-lactamase positive. Stringent protocol such as Antibiotic policy and Hospital infection control program are mandatory to curb these microbes in a tertiary care hospital