ABSTRACT
Immobilized cells of two Chromatium species produced hydrogen continuously for more than 160 hr in 60% and 80% sewage. One strain showing high optimum range of sulfide tolerance (up to 9 mM) produced more hydrogen in 80% sewage while the less sulfide tolerating strain (up to 6 mM) showed hydrogen photoproduction in 60% sewage. Cells were immobilized in alginate and stable hydrogen photoproduction was observed for more than one week. Appropriate strategy necessary for the treatment of sewage and similar industrial effluents for energy reclamation is discussed.
Subject(s)
Chromatium/metabolism , Hydrogen/metabolism , Light , Photochemistry , SewageABSTRACT
A simple kinetic model for the membrane-bound enzyme, 'Na+, K(+)-ATPase', is presented. An equation for the efflux of Na+ and influx of K+ is derived. This also explains multiple ligand binding of homospecies of these cations and competition between heterospecies of these cations. The equation is computed by a small BASIC program and its validity is sought by comparing the results with a few experimental data.
Subject(s)
Computer Simulation , Kinetics , Models, Chemical , Potassium/metabolism , Sodium/metabolism , Sodium-Potassium-Exchanging ATPase/metabolismABSTRACT
The present method involves conversion of the aldehyde produced, as a result of serotonin deamination by monoamine oxidase, to its 2:4 dinitrophenyl hydrazone derivative which gives a stable, bright yellow colour in alkaline solution and can be measured colorimetrically. The derivative is however unstable in the acidic medium and has to be extracted into an organic solvent immediately. The details of the method and its standardization are discussed.