ABSTRACT
Objective To identify the differentially expressed genes of osteoblasts under the stimulation of mechano growth factor E peptide( MGF-Ct24E) and mechanical stress by microarray analysis. Methods Primary osteoblasts were cultured in vitro, which were subjected to mechanical stimulation(with the mechanical strain of 12% and frequency of 0.5 Hz) and MGF-Ct24E treatment(50 mg/L), respectively. The gene expression profiles were analysed by cDNA microarrys and quantitative PCR was used to validate the microarray data. ResultsCompared with the control group, 1 866 genes were found to have differentially expressed in the mechanical loading group, in which 1 113 genes were up-regulated, while 753 genes were down-regulated. 1 178 genes were found to have differentially expressed in the MGF-Ct24E group, in which 796 genes were up-regulated and 382 genes were down-regulated. GO analysis suggested that the gene expression profile of MGF-Ct24E group was consistent with that of the mechanical loading group and differentially expressed genes were mainly involved in cell proliferation and differentiation, response to mechanical stress and mechaotransduction. ConclusionsThe microarray analysis showed that MGF-Ct24E treatment had similar effects with the mechanical loading on the gene expression of osteoblasts, which might provide a novel approach to study the usage of MGF-Ct24E for treating bone repair in the absence of mechanical stimulation.
ABSTRACT
<p><b>OBJECTIVE</b>To explore the influence of Acorus tatarinowii schott on ultrastructure and permeability of BBB.</p><p><b>METHOD</b>The ultrastructure and permeability of BBB on rats with A. tatarinowii by electron microscope were observed. The even's blue (EB) and sodium phenytoin in brain was determined by UV and HPLC-MS.</p><p><b>RESULT</b>After give A. tatarinowii, tight junctions of the endothelial cell opened in cotex, and the concentration of EB and sodium phenytoin in brain are significant increased.</p><p><b>CONCLUSION</b>A. tatarinowii can increase the permeability of BBB, and show its 'Kaiqiao' effect.</p>
Subject(s)
Animals , Male , Rats , Acorus , Chemistry , Blood-Brain Barrier , Brain , Cell Biology , Cells, Cultured , Cerebrovascular Circulation , Drugs, Chinese Herbal , Pharmacology , Endothelial Cells , Microscopy, Electron, Transmission , Neurons , Diagnostic Imaging , Permeability , Rats, Sprague-Dawley , Tight Junctions , UltrasonographyABSTRACT
Objective To analyze the risk factors on type 2 diabetic with foot ulcer.Methods 69diabetic foot patients were involved in the study.According to ulcer,patients were divided into the ulcer group(n=25)and the no-ulcer group (n=44).FBG,2h PBG,HbA1c,TC,TG,HDL,LDL,BUN,Cr.CRP were examined.T-test and chi-square test were used to assess the risk factors on diabetic foot patients with clure.Results Compared with the no-ulce group,the ulcer grou were associated with HDL and CRP.Chi-square-test demonstrated a statistically significant difference between the groups with ulcer and no-ulcer grou on incidence of hypertension and ulcer in diabetic foot patients(P