Correlation between peptidyl arginine deiminase type 4 polymorphism and disease activity in polyarticular juvenile idiopathic arthritis in egyptian patients

Background: juvenile idiopathic arthritis [JIA] is a broad term that describes a clinically heterogeneous group of arthritides of unknown cause, which begin before 16 years of age affecting one or more joints, lasting for at least 6 weeks. PADI4 is one member of PADI gene family. The PADI gene region is located at chromosome 1p36. It codes for enzymes responsible for the posttranslational conversion of arginine residues into citrulline. There are likely to be different genetic risk factors for JIA in different ethnic groups. Therefore, here we sought an influence of HLA-SE and PADI4 on JIA susceptibility in Japanese, because both HLA-SE and PADI4 were reported as significant genetic risk factors for RA independent of ethnicity. Recently, association of PADI4 gene polymorphisms with ACPA positivity and disease activity in polyarticular JIA

Aim of the Work: the aim of this work is to investigate PADI polymorphism rs2240340 to determine whether this polymorphism could be a marker of susceptibility to JIA in Egyptian children and adolescents and whether this single nucleotide polymorphism [SNP] is correlated with clinical parameters in JIA

Patients and Methods: the ethical approval was obtained from the hospital ethical research committee and each patient entering the study will sign an informed consent. Thirty patients included in this study with polyarticular types of juvenile idiopathic arthritis and all of them fulfilled ILAR classification criteria [2004]. All were under the age of sixteen at time of diagnosis. They were recruited from Physical Medicine, Rheumatology and Rehabilitation Department at Al-Hussein and Sayed Galal University Hospitals during the period from January 2018 to June 2018. In this study we measure PADI4 polymorphism and correlate with disease activity in polyarticular JIA in Egyptian patients

Results: association of PADI4 gene polymorphisms with ACPA positivity and disease activity in polyarticular JIA and PADI4 gene polymorphism can be used as a marker of susceptibility to polyarticular JIA

Conclusion: PADI4 gene polymorphism became a marker of susceptibility to polyarticular JIA and gene polymorphism correlated with disease activity in ACPA positivity in polyarticular positive JIA

Age-related response to noise stress on gastric secretion and ulceration in male albino rats

The effects of noise on gastric secretion and ulceration in male albino rats were investigated. Forty-eight rats were used and divided into three main groups i.e. young, adult and senile. Each group was subdivided into control and experimental subgroups. The experimental animals were exposed to noise stress [100 dB] one hour daily for four weeks. The results showed that noise decreased the volume and total acid output in all animal groups and increased the titratable acidity in young group. Noise increased the ulcer index in adult and senile groups and led to vascular congestion, inflammatory cell infiltration, glandular deformity, mucosal atrophy and musculosa hypertrophy in the stomach of all groups

Effect of unsatisfied sexual excitation on reproductive system in male albino rats

Sixty adult albino rats [20 males and 40 females] were used to study the effect of sexual excitation on the male genital system. Rats were divided into two equal groups i.e. control and experimental. Rats were exposed to continuous sexual excitation for one month. Hormonal studies showed imbalance in the pituitary gonadotrophic and corticotrophic hormones as well as testosterone and cortisol. Histological studies showed severe affection of the secretory sex organs [testes, seminal vesicles and prostate] with no significant affection of the penis and vas deference

Effect of Zinc on diabetes mellitus and lipid profile in rat

Ninety-six male albino rats were utilized in this study. They were divided into two main groups; non-diabetic group and diabetic group. The first group was divided into two equal subgroups, one of them was given distilled water and considered as a control group, while the other was given zinc sulphate orally throughout the study period. The other group was divided into equal four subgroups, all of them were diabetics. The first subgroup was served as a diabetic control group. The second subgroup was given zinc sulphate orally. The third subgroup was treated with subcutaneous insulin daily. The last one was treated by both zinc and insulin simultaneously. Diabetes mellitus was induced by intraperitoneal injection of alloxan monohydrate solution. All rats, in the previously mentioned groups, were fasted for 12 hours before taking blood samples for performance of oral glucose tolerance test, calculation of KG value and estimation of lipid profile. Induction of diabetes mellitus by alloxan was associated with significant increase in fasting scrum glucose and significant decrease in the KG value when compared with the corresponding values in non-diabetic control group. Also, alloxan-induced diabetes mellitus in rats produced significant increase in fasting serum cholesterol, fasting serum triglycerides, fasting serum LDL and significant decrease in fasting serum HDL when compared with the corresponding values in non-diabetic control group. The ratio of HDL to total cholesterol significantly decreased when compared with the corresponding value in non-diabetic control group

Effect of bilateral nephrectomy on intestinal motility in albinoi rats

The frequency as well as the peak tension of isometric contractions of loops of small intestine isolated from rats three days after bilateral nephrectomy as well as those of sham controls were assessed. Intestinal loops isolated from rats subjected to bilateral showed a significant inhibition of low frequency contractions as well as the peak tension. On the other hand, the high frequency contractions were not altered compared with sham controls. The induced lesion in intestinal motility particularly in the low frequency contractions controlled by enteric nervous system could be due to enteric neuropathy. These data represented direct evidence for the disordered intestinal motility in albino rats subjected to bilateral nephrectomy