ABSTRACT
The purpose of this study was to evaluate the effects of d-glucosamine sulfate on tem-poromandibular joint arthritis. The present study was carried out on ninety male albino rats of average weight [150 - 20gmO]. These rats were divided into control healthy, arthritic arthritic nontreated and treated groups. Thirty rats for control and sacrificed parallel with group II and, III, thirty rats for group II [arthritic nontreated] which subdivided into three equal subgroup A, B,C they were sacrificed after arthritis induction by thirty, sixty, ninety days respectively and thirty rats for group III [treated group] which subdivided into three equal subgroups D, E, F. they were sacrificed after thirty, sixty and ninety days of treatment of arthritis respectively. The specimens were taken, fixed, demineralized and processed for paraffin sections. The sections were studied histologically, his-tochemically, statistically and digital image analysis.The results of this study, histologically; in group II there were side adhesion between the articular cartilage and the disc, thinning and irregularities of some parts of articular cartilage and destruction of subchondoral bone. These criteria decreased in severity from subgroup A to C. but in group III all these criteria in group II regenerated gradually from subgroup D to F which appeared nearly normal at subgroup F. Histochemically; in group II the collagen fibers were decreased and disarranged with matrix defect and loss of toludine blue stain, intense reaction to acid phosphatase, mild reaction to alkaline phosphatase. In group III the collagen fibers and matrix regenerated gradually from D to F after the treatment, very weak reaction to acid phosphatase and intense reaction to alkaline phosphatase. Digital image analysis revealed that the net collagen matrix was increased in group III more than group II. statistical analysis, there was significant increase in cartilage thickness and collagen matrix in group III more than group II.Based on the previous results we conclude that, treatment of temporomandibular joint arthritis by d-glucosamine sulfate leads to decrease the degenerative changes of osteoarthiritis in the joint, increases collagen formation, enhances the matrix formation and reduce the osteoarthritic pain. This paper was extracted from a thesis submitted in partial fulfillment for master degree in oral biology by rehab r elzehery [demonstrator in oral biology, faculty of dentistry, man-soura university]
Subject(s)
Animals, Laboratory , Temporomandibular Joint/pathology , Treatment Outcome , RatsABSTRACT
The aim of this study was to examine the effect of diclofenac sodium on the submandibular salivary gland through the light microscope [using H and E stain] and the transmission electron microscope. Light microscopical examination revealed structural degeneration and vacuolization of acinar cells in diclofenac sodium treated rats, with dilatation of the ductal lumina and apparent chronic inflammatory cells infiltration together with red blood cells extravasation. The examination of the submandibular salivary glands of treated rats, in electron microscope, revealed the serous acinar cells with vacuolated cytoplasm and few cytoplasmic organelles. There was extensive coalescence of the mucous secretory granules and the lumina of the mucous acini were abnormally distended and filled with the mucous secretory materials. In both serous and mucous acinar cells, the mitochondria were few and altered, while the rough endoplasmic reticulum was significantly inconspicuous. The nuclei were heterochromatic with irregular nuclear outlines
Subject(s)
Animals , Male , Submandibular Gland/anatomy & histology , Submandibular Gland/cytology , Diclofenac , Prostaglandins , RatsABSTRACT
The present investigation was undertaken on rats to demonstrate the structural changes that took place after the exposure of the gingiva to a dental visible light curing unit. Sixteen rats were irradiated and four were considered as controls. The mandibular right first molar was exposed to radiation for 40 seconds. The animals were classified into four groups [4 experimental and 1 control] and were sacrificed immediately, 2 days, 4 days and 2 weeks after exposure. Specimens were processed for ultrastructural and light microscopic investigations. The results indicate that emission from dental light curing units can affect the oral mucous membrane and may reduce its functional abilities