Pharmaceutical and clinical evaluation of intravenous admixture of dobutamine and dopamine used in treatment hypotension in neonatal intensive care units

The aim of this study was to evaluate compatibility and stability of the maximum concentration used for binary admixture containing dobutamine and dopamine in 5% glucose. The maximum concentration of each drug was 5.76 mg/ml of dobutamine and 2.88 mg/ml of dopamine in 50 ml of 5% glucose. The physical compatibility of binary admixtures was assessed using visual inspection and pH determination immediately after preparation [at 0 time] and after 24 hrs. The chemical stability was assessed using high performance thin layer chromatoghraphy [HPTLC]. The method is based on HPTLC separation of the two drugs followed by densitometric measurements of their spots at 254 nm using Camag TLC Scanner 3. The mobile phase comprised ethyl acetate: n-propanol: water: glecial acetic acid [60:24:9:3, v/v/v/v]. The results revealed that no precipitation, gas evaluation, color change, pH change or chemical incompatibility were observed over the entire time of mixing of two drugs in 5% glucose solution

Compatibility and stability of ternary admixture of midazolam, dobutamine and dopamine in 5% glucose or 0.9% sodium chloride solution

The aim of this study was to evaluate compatibility and stability of the maximum concentration used for ternary admixture containing midazolam, dobutamine and dopamine in 5% glucose and 0.9% sodium chloride solutions. The maximum concentration of each drug was 0.144 mg/ml of midazolam, 5.76 mg/ml of dobutamine and 2.88 mg/ml of dopamine in 50 ml of 5% glucose or 0.9% sodium chloride solutions. The physical compatibility of ternary admixtures was assessed using visual inspection and pH determination of ternary admixtures immediately after preparation [at 0 time] and after 24 hrs. The chemical stability was assessed using high performance thin layer chromatoghraphy [HPTLC]. The method is based on HPTLC separation of the three drugs followed by densitometric measurements of their spots at 254 nm using Camag TLC Scanner 3. The mobile phase comprised ethyl acetate: n-propanol: water: glecial acetic acid [60:24:9:3, v/v/v/v]. There were no visual changes [such as precipitation, gas evaluation or change in color] during 24 hrs after preparation of admixture. Also, there was no change in pH values of admixtures during that time. The results revealed chemical stability of midazolam, dobutamine and dopamine over the duration of mixing [24 hrs] in 5% glucose or 0.9% sodium chloride solutions

comparison of transcutaneous and total serum bilirubin in Egyptian neonates

Previous studies have suggested that transcutaneous bilirubinometry [TcB] may provide a useful method for screening for significant jaundice, thereby reducing unnecessary blood tests. The present study aimed to evaluate the validity of TcB versus serum bilirubin [TSB] measurements in Egyptian full term babies. Babies born at more than 34 weeks gestation who had not previously been exposed to phototherapy and were requiring blood sampling in the first week of life were inducted in the study. TcB measurements were made at the same time as. SB for 150 neonate [57% males and 43% females]. There was a significant positive correlation between SB and TcB measurements [n = 150, p < 0.0001] and TcB was more accurate in serum bilirubin level more than18mg/dl. Bilirubin can be measured accurately by TcB, but before hospital admission or exchange transfusion, SB should be done. Further studies should be done on neonates with different skin color and preterm babies

Seropositivity of chlamydia trachomatis among saudi pregnant women in Makkah

To determine the seroprevalence rates of immunoglobulin G [IgG] and immunoglobulin M [IgM] to Chlamydia trachomatis in Saudi pregnant women. Using enzyme-linked immunosorbent assay [ELISA], a total of 1600 serum samples were tested for antibodies to Chlamydia trachomatis known to cause a variety of clinical syndromes in women and newborn infants. Chlamydia trachomatis IgG antibodies were detected in 8.7% and IgM antibodies were found in 1.5% of different age groups Pregnant Saudi women have low prevalence rate of Chlamydia trachomatis IgG antibodies and lower prevalence for Chlamydia trachomatis IgM