ABSTRACT
Oral cancer prevention using naturally occurring substances that could be included in diet consumed by humans, is gaining attention. Investigating the chemopreventive effect of green tea, through assessment of mutant p53 immunoexpression, in the hamster buccal pouch epithelium-induced carcinogenesis. Forty-five hamsters were divided into 3 groups. Group A was served as controls. In group B, left pouches of hamsters were painted with 0.5% Dimethylbenz-[a]-anthracene [DMBA], 3 times/week for 6 weeks. Hamsters of group C were given Epigallocatechin Gallate [EGCG]; five animals were sacrificed [Group C3], the remaining 20 were divided into 2 groups; group C1 was given EGCG and DBMA and group C2 was given DBMA only. All pouches were surgically excised, fixed, processed for H and E and mutant p53 immunohistochemical staining. Mutant p53 immunoexpression score was highly significant in group B, compared to group C1 which was given EGCG only. Administration of EGCG, before and in combination with the carcinogen [group C1] resulted in significantly decreased expression of mutant p53 parallel to decreased grades of dysplasia. Administration of EGCG alone for 2 weeks [group C3] showed negative mutant p53 expression. EGCG proved to be a chemopreventive and/or protective agent; through suppressing and/or retarding malignant transformation, reducing cell proliferation and inducing apoptosis. The apoptosis may be possibly through preventing p53 mutations
Subject(s)
Animals, Laboratory , Mouth Neoplasms/pathology , Cricetinae , Protective Agents , Tea , Genes, p53 , ImmunohistochemistryABSTRACT
A serum-free medium [SFM] was evaluated for the growth of bovine turbinate [BT] cells used for the production of Sarcocystis falcatula merozoites. Serum free cultures used to propagate S. falcatula were compared to cultures maintained in media supplemented with fetal calf serum [FCS] or horse serum [HS]. Serum free cultures were more effective and very promising than the others in supporting the proliferation of S. falcatula merozoites. However, the serum free cultures were unable to adequately support BT cell proliferation compared to the serum-supplemented cultures. No significant differences were seen between cultures supplemented with HS or FCS used for the production of S. falcatula merozoites or BT cells. The rate of BT cell proliferation in response to SFM and different media supplements was assessed in a 96-well plate format using methylene blue staining assay. This technique was superior to manual counting method and allowed quick and accurate quantitative comparison between the response of proliferating BT cells to different growth conditions
Subject(s)
Culture Media, Serum-Free , Methylene BlueABSTRACT
Antioxidant enzymes work together in human blood cells against toxic reactive oxygen species. Recently, oxidative stress has been linked to the pathogenesis of several chronic inflammatory disorders of the respiratory tract. However, little is known about the connection between oxidative products and antioxidant enzymes in rhinoscleroma. Therefore, this study was designed to investigate and to determine the level of lipid peroxidation, nitric oxide and antioxidant enzyme activities in blood of patients suffering from rhinoscleroma. The study was carried out on 33 patients and 15 normal healthy controls. Venous blood samples were taken from all subjects. The specific activity levels of malondialdehyde [MDA] and nitric oxide [NO] as oxidant enzymes, superoxide dismutase [SOD], and catalase [CAT] as antioxidant enzymes were estimated in all blood samples. Levels of MDA, and NO were significantly higher [P <0.001], whereas levels of SOD, and CAT showed no significant change [P >0.05] in rhinoscleroma patients when compared with control group. These results provide some evidences for potential role of oxidative products as contributing factors in patients with rhinoscleroma, and estimation of antioxidant enzymes in blood may, therefore, help to orientate rhinoscleroma therapy