Effect of follicular flushing during oocyte retrievalon clinical outcome of assisted reproductive technology

Is to determine whether follicular aspiration and flushing increase the number of oocytes yield and pregnancy outcome over aspiration alone in women undergoing ICSI. Prospective randomized controlled trial. One hundred eighty five infertile women who underwent ICSI were included in the study. They were randomized into two groups 92 cases in [aspiration and flushing group] and 93 cases in [aspiration only group],during the period from September 2011 to September 2013. Controlled ovarian hyperstimulation using long GnRH agonist was the standard protocol, hCG administrated 10000 iu when three or more follicles were at least 18 mm in largest diameter, Trans-vaginal follicular aspiration performed 34-36 hours after hCG trigger. In the aspiration alone group, a 16 gauge single lumen needle used, with suction continue until a small amount of blood stained fluid appeared in the tubing or flow stop, When flushing accompany aspiration of follicular fluid in the study group, the same needle used with a double-way tap allowing flushing of [2 ml] of embty follicleby Earl's medium till oocyte retrieved or maximum two times. The study observed 60.5 % oocyte recovery rate with aspiration only compared with 80.9 % with follicular aspiration and flushing.Operative time [minutes] was significantly longer among flushing group, the retrieval time was 1.3 fold higher among those undergoing follicular flushing. Pregnancy was non-significantly more frequent among flushing. Implantation rates non-significantly more frequent among flushing group than non-flushing group [31.6% versus 26.3%, P= 0.424] and ongoing clinical pregnancy non-significantly more frequent among flushing group [27.4% versus 21.1%, P= 0.31]. Conclusion, flushing non-significantly increase implantation and clinical pregnancy outcome and associated with a significant increase in the procedure time for oocyte retrieval, so patient groups where a small number of oocytes are available for retrieval may represent patients most likely to benefit from follicle flushing as only one extra oocyte may affect the outcome

Value of morrow procedure in surgery of discrete membranous subaortic stenosis

Subuortic stenosis constitutes up to 20% of all farms of left ventricular outflow had [LVOT] obstruction in children. Although the resulting significant left ventricular hypertrophy and aortic insufficiency are clear indications for surgery, controversy persists whether to remove the membrane in symptom-free patients with an isolated membrane or not. In addition to the timing of surgical repair, the surgical technique remains an area of discussion. In this study, we try to evaluate the impact of early surgical interference as well as surgical technique on the early and mid-term outcome. 44 patients with a mean age of 9.26 years [range, 2.4-28 years] underwent surgical resection for discrete membranous subaortic stenosis [DMSS]. For the purpose of the study, the patients were divided into two groups. Group I consisted of 31 patients who underwent membranectomy combined with routine transoortic myectomy. Group II consisted 13 patients to whom only excision of the subaortic membrane was done [membranectomy] without any myotomy or myectomy. Group II was done in Assuit University Hospital between 2001 and 2003. Group I was done in Paediatric cardiothoracic surgery unit [Children Hospital. Assuit University] between 2009 and 2013, in early postoperative results, the mean LVOT gradient in group I was statistically significantly lower than that of group II [p=0.000]. Aortic regurge [AR] improved in all patients of group I but progressed in five patients of group H. Mid-term follow up showed better results in group I than group II early resection of DMSS, before development of significant left ventricular hypertrophy and aortic insufficiency is advisable. Resection of the obstructing membrane together with generous myectomy of the LVOT is a safe and effective treatment in terms of more significant reduction of LVOT gradient; both early and in mid-term follow up, with lower incidence of recurrence and significant improvement of associated aortic incompetence