Larvicidal potency of some extracts from wild Egyptian plants to Culex pipiens

Out of fifty Egyptian plant extracts tested against the 4th larval instar of Culex pipiens, seven extracts possessed insecticidal activity. Extracts of Lyciums shawii was the most toxic [LC50, 45.76 mg/l]. Other active extracts were these of Ononis vaginalis [58.2 mg/l], Ranunculus sceleratus [91.11 mg/l], Ammi majus [109.5 mg/l], Salvia lanigera and Moricandia nitens [126.9 mg/l], and Alkanna tinctoria [138.5 mg/l]. The rest of the plant extracts were less or non-active at 100 mg/l

simple method for production of specific antibodies to carbaryl

Succinated derivatives of carbaryl were firstly prepared. The succination process was achieved by incubating the carbaryl with succinic anhydride in pyridine at room temperature for 3 days. The carbaryl derivatives were then coupled covalently to bovine serum albumin [BSA] by use of carbodiimide condensation and the reaction was carried out in aqueous pyridine. The protein-carbaryl-derivative binding was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis [SDS-PAGE] and UV absorbance. The carbaryl BSA conjugates were injected several times in rabbits and the obtained sera were checked for titer affinity and specificity using immunodiffusion technique. From the obtained results, it can be concluded that this method could be used as a simple and useful way for successful achievement of carbaryl-conjugate antibodies production