Enzyme inhibition, antioxidant and antibacterial potential of vasicine isolated from Adhatoda vasica Nees

Vasicine [1] was isolated from the ethanolic extract of Adhatoda vasica Nees [Acanthaceae] and the structure was confirmed using spectroscopic techniques. Acetylcholine esterase, trypsin, DPPH inhibition potential and FRAP assay were carried out using in vitro models. The results showed 38.4 +/- 1.2% and 37.4 +/- 1.1% activity in acetylcholine and trypsin inhibition assays respectively. The compound [1] exhibited significant DPPH inhibition activity [70.4 +/- 1.3%, IC[50] = 212.3 +/- 1.9 microM]. A dose dependant behavior of vasicine [1], was indicated in the FRAP assay. Antibacterial activity was checked according to agar well diffusion assay and results revealed that vasicine [1] showed moderate activity