ABSTRACT
Background: sperm processing methods separate motile sperms with good morphology from dead and abnormal forms of sperms, immature germ cells, and non-sperm cells
Objective: the propose of this study was to compare the efficacy of upstream and swim-up processing techniques to separate sperms with the high quality especially in relation to sperm chromatin integrity
Materials and Methods: this experimental study used semen samples from 60 normozoospermic men. Specimens were divided into equal aliquots for processing by swim up [group A], and upstream [group B] methods and compare with control by raw semen [group C]. Sperm concentration, morphology, motility, DNA fragmentation and chromatin maturation were measured in these three groups
Results: the results revealed that sperm concentration in the swim up samples was significantly greater than upstream samples [p/= 0.4]. In addition, sperm DNA fragmentation and chromatin maturation were not significantly different between the three groups [p >/= 0.1]
Conclusion: according to results, apparently the upstream method had no significant efficiency to separate good quality sperms compare to swim up. Therefore, swim up seems to be a simple, inexpensive, reliable and widely available method with an efficient yield to separate motile sperm with good morphology and better chromatin integrity for insemination in the infertility clinics
ABSTRACT
Background: Endometrium undergoes several changes in structure and cellular composition during pregnancy. Granulocyte Colony-stimulating Factor [GCS-F] is an important cytokine with critical role in embryo implantation and pregnancy. The aim of the present study was to evaluate the impact of intrauterine injection of G-CSF in patients who suffer from unexplained recurrent miscarriage [RM]
Methods: In the present randomized clinical trial, a total of 68 patients were randomly allocated into two study groups including intrauterine G-CSF [n=23, 300mg] injection and control group [n=27, no G-CSF injection]. Eighteen out of 68 patients were excluded from the final analysis due to different reasons. All patients were in Ovulation Induction [I/O] cycle. In G-CSF group, intrauterine injection of G-CSF was done twice in the cycle. All enrolled patients were under 40 years old and had at least two unexplained pregnancy losses. Pregnancy was evaluated by titer of bhCG, presence of gestational sac [implantation] and fetal heart rate [clinical pregnancy] was assessed by vaginal ultrasonography. Student's T test and Mann-Whitney U were used for analysis. The p
Results: No significant differences were observed between the two study groups when the rates of chemical pregnancy [26.1%vs.29.6%, p=0.781], implantation [26.1%vs.22.2%, p=0.750], clinical pregnancy [17.4% vs.11.1%, p=0.689] and abortion [33%vs.37.5%, p=0.296] were compared
Conclusion: In our study, no significant difference was observed between the two study groups when the rates of chemical pregnancy, implantation, clinical pregnancy and abortion were compared
ABSTRACT
Nuclear transfer procedures have been recently applied for clinical and research targets as a novel assisted reproductive technique and were used for increasing the oocyte activity during its growth and maturation. In this review, we summarized the nuclear transfer technique for germinal vesicle stage oocytes to reconstruct the maturation of them. Our study covered publications between 1966 and August 2017. In result utilized germinal vesicle transfer techniques, fusion, and fertilization survival rate on five different mammalian species are discussed, regarding their potential clinical application. It seems that with a study on this method, there is real hope for effective treatments of old oocytes or oocytes containing mitochondrial problems in the near future