ABSTRACT
Objective: To investigate the effects of diet-induced obesity on the expression of nuclear factor-KB [NF-KB] and visfatin messenger RNA in male Wistar rats' tracheae after sensitiza-tion with ovalbumin [OVA]
Materials and Methods: Twenty male Wistar rats were divided into 4 groups [n = 5 for each group], which included a control group fed a normal diet [ND] and groups fed normal diet, OVA-sensitized [S+ND]; high-fat diet [HFD] only [diet-induced obesity]; and high-fat diet, OVA-sensitized [S+HFD]
All animals were fed for 8 weeks with standard chow or a high-fat diet, and then were sensitized and challenged with OVA or saline for another 4 weeks as per the above groups. The rats were anesthetized, after which the necks were exposed and the tracheae iso-lated and examined for expression levels of NF-KB and visfatin mRNA with the real-time polymerase chain reaction method. Data were compared between the different groups using one-way analysis of variance
Results: The expression level of NF-KB mRNA in the S+HFD group was 2.67, which was statistically higher than the levels in the ND [0.96; p = 0.001], S+ND [1.86; p = 0.05], and HFD [1.26;p = 0.001] groups. Also, the visfatin mRNA expression level in the S+HFD group was 4.21, which was higher than the levels in the ND [0.92], S+ND [1.79], and HFD [2.20] [p = 0.001] groups
Conclusion: In this study, the expression levels of NF-KB and visfatin were markedly higher in the S+HFD group in comparison to the other groups. These findings indicate that alternative signaling pathways might be activated in diet-induced obesity as-sociated with the OVA-sensitized animal model and could be responsible for possible altered sensitization phenotype
Subject(s)
Animals, Laboratory , NF-kappa B , Rats, Wistar , Obesity , Real-Time Polymerase Chain Reaction , Asthma , RNA, Messenger , Ovalbumin , TracheaABSTRACT
Background and Objectives: helicobacter pylori prevalence is high in developing countries. This microorganism is accepted as the most important agent of gastritis and as a risk factor for peptic ulcer disease and gastric adenocarcinoma. Currently many diagnostic methods exist for detecting H. pylori, however they all have limitations. The aim of this descriptive study was to evaluate diagnostic methods, such as rapid urease test, culture and polymerase chain reaction [PCR]
Materials and Methods: for identification of Helicobacter pylori 100 patients [46 women and 54 men] who were suffering from digestive complaints and referred to the endoscopy department of Hajar Hospital in Sharkord were participated in the study. Gastric biopsy samples were collected from each patient, then polymerase chain reaction, culture and rapid urease test were performed. DNA extraction was followed by PCR was used for diagnosis of ureC gene
Results: biopsy samples from100 patients were evaluated. H. pylori was positively identified by PCR in 78/100 [78%] of the patients, while positive samples were found in 52 [52%] and 48 [48%] of the patients by RUT and culture methods, respectively. In results of the culture, there was an agreement of 100% with PCR
Conclusion: three different methods for the detection of Helicobacter pylori were evaluated. Given that the PCR test has higher sensitivity and specificity to detect H.pylori comparing rapid urea as test, therefore this method could be used to detect H.pylori
ABSTRACT
Various drugs are administered intra-articularly to provide postoperative analgesia after arthroscopic knee surgery. The purpose of this study was to assess the analgesic effects of intra-articular injection of a dexmedeto-midine following knee arthroscopy. Forty six patients schadualed for arthroscopic knee surgery under general anaesthesia, were randomly devided into two groups. Intervention group received 1microg/kg dexmedetomidine [D] and isotonic saline. Control group received 25ml isotonic saline [P]. Analgesic effects were evaluated by measuring pain intensity [VAS scores] and duration of analgesia. There was no significant difference between the two groups in terms of age, sex and weight. The mean of post-operation pain severity in 1, 3, 6,12, and 24 h was significantly lower in the intervention group [D] in comparison w] to toe contio] group [P]. toe mean of toe tola] dose oftramado] consumption was significantly lower in the mterven-tion group in comparison with the control group [P<0.001]. Intra-articular injection of dexmedetomidine at the end of arthroscopic knee surgery, alleviates the patients' pain; reducing the postoperative need for narcotics as analgesics, and increase the first analgesic request after operation
ABSTRACT
The aim of present study was to determine the effectiveness of conceptual map training method on the achievement of social-economic skill course in male students with intellectual disability. This study was semi-experimental and 34 male students with intellectual disability who were educating in 3rd grade at pre-professional level in high school selected randomly from Talash Exceptional Center in Tabriz City. Their academic achievement in social-economic skill course were assessed by teacher-made and performance tests. Subjects were assigned in control and experimental groups randomly and equally. Experimental group participated in 8 sessions and were trained by conceptual map method for one month [8 sessions; 2 sessions weekly]. At the end of eightieth session and one month later, subjects answered to teacher-made and performance tests again. Data were analyzed by analysis of covariance. Findings showed that the conceptual map training method had positive effect on achievement of social-economic skill in students [p<0.01]. But, its effectiveness wasn't persistent after one month in follow-up test. Regarding to positive effects of conceptual map training method on meaningful learning, it seems as an effective method for intellectually disabled male students who require deep learning to understand the content of their lessons