ABSTRACT
Euphorbia milii is a Pakistani herb used for various infectious diseases. In this study we have carried out phytochemical, antibacterial and antioxidant investigation of different extracts/fractions. Phytochemical studies showed the presence of cardiac glycosides, steroids/phytosterols, anthocyanin, proteins, terpenoids, flavonoids and tannins. Susceptibility testing by well diffusion assay of its chloroform and methanol fractions revealed good antimicrobial activity against Klebsiella pneumonia and Staph epidermis. Ethyl acetate fraction of roots also exhibited considerable antimicrobial activity against most of tested pathogens. Various fractions [Hexane, chloroform, methanol and water] of E. milii were screen for their antioxidant potential using DPPH radical scavenging assay at different concentrations among these, chloroform fraction exhibited good scavenging activity. The IR spectroscopy of the various extracts/fractions indicated the presence of OH, saturated CH stretching, C=C, C=O, NO[2], C-N, Ar-O, C-O- and R-O-Stretching respectively. The findings provide helpful evidence for the use of E. milii in traditional medicines
ABSTRACT
Crude powder as well as the ethanolic extract of Ajuga bracteosa were given dialy orally to rabbits for a period of 4 days. Highly significant increase in the serum cholesterol, total lipid and glucose level was observed in case of both powder and the extract. The effect of powder on these parameters was more pronounced as compared to the ethanolic extract. Serum total protein, uric acid, urea and enzymes ALT and AST showed a remarkable decrease after the treatment of rabbits