Analysis of Amantadine Hydrochloride-Phenyl Isothiocyanate Complex in Bulk and Pharmaceutical Dosage Forms by RP HPLC-PDA Method.

Aim: The aim of the present investigation was to perform the precolumn derivatization of Amantadine Hydrochloride (AMT) with phenylisothiocyanate and to develop a RP-HPLCPDA method for the quantification of Amantadine Hydrochloride-phenylisothiocyanate (AMT-PITC) complex in bulk and dosage forms which is rapid, sensitive and economical. Study Design: Method development and Validation study. Methodology: A Phenomenex C18 RP column of 250 x 4.6mm dimensions and 5μm particle size with mobile phase containing water and acetonitrile (40:60% v/v) was used at isocratic mode binary pump and eluent was monitored at 273nm. Results & Discussion: The retention time of AMT-PITC complex was 6.3 min. The developed method showed a good linearity in the concentration range of 10-50μg/mL with a correlation coefficient >0.998. The recoveries ranged between 95-105% with a Relative Standard Deviation of (RSD) < 2%. Conclusion: The developed method was validated as per ICH guidelines and successfully used for quantification of AMT by derivatization with PITC. The method was found to be rapid, specific and accurate.

Determination of left ventricular mass by echocardiography in normal Arab people

Echocardiographic data from 168 normal [18 0.05]. In female [N=86] the LVM value was 168 +/- 17 grams [mean +/- SD]. Data also showed significant correlation of LVM with weight, body surface area and also with age [p<0.05] but not with height. Our results demonstrated that values of LVM in the Arab people are not different from values obtained in other parts of the world including Europe and North America. More studies are still essential in different Arab countries

Acute effects of a single short exercise on glucose, cholesterol and triglyceride levels in healthy, diabetic and patients with hyperlipidemia

Plasma glucose, serum triglyceride and serum cholesterol levels were determined before and immediately after a single exposure to short term exercise in normal healthy subjects and in patients with hyperlipidemia and patients with diabetes mellitus. The first group is a control group consisting of 25 male normal subjects with a median age of 44.0 years. The second group consists of 14 male hyperlipidemic paients with a median age of 41.0 years. The third group consists of 14 male diabetic patients with a median age of 44.5 years. The study showed a significant in the triglyceride and cholesterol levels with no change in the glucose levels immediately after exercise in the diabetic group. Although there was a mild increase in the post-exercise levels of these substances in the control group, this increment was statistically not significant. Again, there was a mild rise in the glucose and triglyceride levels and a mild decrease in the cholesterol level in the post exercise period in patients with hyperlipidemia but these changes were statistically not significant