ABSTRACT
Histological typing, especially the evidence of anaplasia and stage of the tumor are two major prognostic indicators in Wilms tumor, but some genetic factors have recently been noted. Mutations in TP53, which is a tumor suppressor gene, have been demonstrated to be associated with poor prognosis in some malignancies. There are also few studies which have confirmed the correlation between p53 protein overexpression and advanced course in Wilms tumor. This study was conducted to determine the correlation p53 immunoexpression and prognosis and outcome of Wilms tumor. The overexpression of p53 in 44 specimens of children [26 boys and 18 girls] with Wilms tumor [median age, 36 months; range, 4 to 96 months] was assessed in Mofid Children's Hospital, Tehran, Iran. The overexpression of p53 was determined by immunohistochemistry with antihuman p53 antibody. A positive immunostaining for p53 was detected in 24 of the 44 cases [54.7%]. The grade of the tumor and anaplasia were associated with p53 positivity, but there was no association of p53 with the tumor stage. In comparison to p53 negatives, week-moderate and high p53-expressing tumors had significantly decreased the overall survival [hazard ratio, 3.75 and 8.61; P=.05 and P=.01, respectively]. Overexpression of p53 protein in Wilms tumor is an indicator of poor pro gnosis, because it is correlated with unfavorable histology tumors and a shorter survival period
ABSTRACT
Several investigations have shown positive effects of microcurrent electrical current on fracture healing. In this study, the effects of microcurrent [microampere] on the full thickness incisional wound healing of rabbits were evaluated by histological and tensiometrical methods. Thirty male adult rabbits were randomly divided into control and experimental groups. Each group divided into 3 subgroups, based on duration of study [4,7,15 days]. Under general anesthesia and sterile conditions, one full thickness incision on skin of each rabbit was made. From the day of surgery, the experimental group received electrotherapy daily for 2 hours [current intensity: 200 micro A/cm [2], current density: 66 micro A/cm [2] frequency 0.5 Hz]. Polarity was negative at first 3 days and was positive thereafter. At the end, rabbits were killed by choloroform and 2 samples were obtained from the wound tissue and the adjacent normal skin for histologic and tensiometeric studies. Number of neurtophils and fibroblasts and cross sections of vessels were counted. Data were analysed using student t test. The number of fibroblasts on seventh day [862.6 +/- 70.1] was significantly higher [P<0.05] than control group [468 +/- 2+59]. Tensile strength of experimental group at fifteenth day [2138.2 +/- 212] was significantly higher than relevant control group [1443.1 +/- 218.8]. Daily application of microcurrent electrical current significantly accelerated wound healing process of incisional wound of skin of rabbits
Subject(s)
Animals, Laboratory , Electric Stimulation Therapy , Wounds and Injuries , Rabbits , SkinABSTRACT
Background: While considering the rise in the mean age of the chronic renal failure [CRF] patients and increasing frequency of those who need constant hemodialysis, the creation of a native arteriovenous fistula [AVF] is not possible, which could be due to the inaccessibility of the superficial veins. This study was conducted to evaluate the efficacy of the vascular access, using cryopreserved jugular vein
Materials and Methods: In this experimental study, 15 sheep [Wt.= 30-40 kg] were selected between the year 2001 and 2002. The external jugular vein of each sheep was excised and after cryopreservation was put in liquid nitrogen [-196°C]. Twenty-eight days later, the sample veins were used as allograft [in another sheep], and autograft [in the same sheep] and were placed between carotid artery and external jugular vein as bridge AVF. The efficacy, function, and patency were evaluated using doppler sonography and pathologic report
Results: Patency rate of sample was 100% after 3 months in both allografts and autografts. Thrombosis, hematoma, and infection were not seen. No inflammation was detected in pathologic report
Conclusion: This study showed that using allograft vein for vascular access is feasible and suitable, and it is recommended specially for those with previously infected fistula
ABSTRACT
Introduction: This paper presents the results of a study on the effects of two different doses of low-power laser irradiation on healing of deep second degree burns
Material and Methods: 60 rats were randomly allocated to one of four groups. A deep second-degree burn was inflicted in each rat. In the control group [CG] burns remained untreated; in Groups LG1 and LG2 the burns were irradiated with low-power Helium Neon laser with energy densities of 1.2 J/cm[2] and 2.4 J/cm[2] respectively. In the fourth group [G4] the burns were treated topically with 0.2% nitrofurazone cream. The response to treatments was assessed histologically at 7, 16 and 30 days after burning and microbiologically at Day 15
Results: The number of macrophages and the depth of new epidermis was significantly less in the laser treated groups compared to control and nitrofuorazone treated groups. Staphylococcus epidermidis was found in the wounds of all rats in the laser treated groups
Conclusion: Irradiation of deep second-degree burn with low-power laser produced no beneficial effects on healing of burns