Effect of whey permeate and yeast extract on metabolic activity of Bifidobacterium animalis subsp. lactis Bb 12 cultivated in skim milk based media

In fermented products containing Bifidobacteria, factors such as organic acid concentration and beta-galactosidase activity are important in the development of flavor and texture of final products. Both the process conditions and medium components have significant effects on fluctuation of such factors. The effects of whey permeate powder and yeast extract concentrations, as nitrogen sources was investigated, on metabolic activity of Bifidobacterium animalis subsp. lactis Bb 12 in skim milk based media. Development of organic acids, growth rate and beta-galactosidase activity under uncontrolled pH conditions was also monitored. Media with high concentrations of nitrogen sources showed maximum viable counts of B. animalis. The activity of beta-galactosidase increased during the logarithmic phase and the initial stage of the stationary phase of growth and then decreased thereafter. Increasing the yeast extract concentration resulted in an increase in the specific sugar consumption rate with concomitant reduction in formation of acetic acid and formic acid. Consequently, the molar ratio of acetic acid to lactic acid was decreased. Using limited amounts of nitrogen sources resulted in more organic acid production in the tested microorganism. During the early hours of fermentation in which the amounts of nitrogen levels were limited, the specific rate of beta-glactosidase activity was very high. Therefore, by evaluating the activity of this enzyme we can estimate the amount nutrient components in the medium.The production of succinic acid was observed during logarithmic and stationary phase in all fermentation media. Citric acid that naturally exists in whey and skim milk powder was consumed during the stationary phase of growth by B. animalis and its consumption correlated significantly with the production of succinic acid at this stage

Intermediate production of mono- and diolein by an immobilized lipase from Candida antarctica

Lipase from Candida antarctica, fixed on macroporous acrylic resin, has been used for the intermediate production of mono- and diolein by hydrolysis of triolein. The effect of altering concentrations of triolein and glycerol and the function of the molecular sieve on the hydrolysis reaction of triolein were investigated. The highest hydrolysis yield was observed for the utmost concentration of triolein, which gave a hydrolysis optimum at the lower reaction time. Raising the concentration of triolein resulted in a 13.7 times increase in mono-and diolein during reaction period. Addition of glycerol to the reaction mixture had a considerably higher positive effect on the production of monolein than that of diolein. The use of a molecular sieve in the mixture was found to be the most effective environment tested, which demonstrated high activity and excellent selectivity toward the formation of intermediate monolein