ABSTRACT
Coagulase-negative Staphylococci [CoNS] which are indigenous flora of our skin and mucous membrane were isolated in large numbers from cases suffering from eye diseases as chronic blepharitis, purulent conjunctivitis, suppurative keratitis and corneal ulcer. This study determines CoNS pathogenicity in eye infections in patients at Ophthalmic Centre, Mansoura University. Total of 40 isolates of CoNS from infected eyes and 3 isolates from healthy eyes [control] were identified to the species level using automatic Sensititre, subjected to antibiotic susceptibility testing, Plasmid DNA analysis, B Lactamase production, slime production, and detection of isolates icaA and icaD genes by PCR. S. epidermidis were identified in 79% of isolates. Other species of CoNS were identified in 21% of isolates [P <0.001]. 95% of CoNS were resistant to pencillin and 95% of them were sensitive to vancomycin and teicoplanin. 62.7% were beta-Lactamase producer, 20.9% were Plasmidless. There were10 groups of different plasmid profile and 27.9% contained plasmids of MW 14MDs. 47% of S. epidermidis were potitive for slime production and 52.9% of S. epidermidis were positive ica A and D genes. Slime positive isolates were multidrug-resistant as compared to slime negative isolates [P <0.001]. Slime-producers possessed multiple plasmids in contrast to no slimeproducers [P<0.001]. Plasmid of MW14MDs had correlation with slime producer and with multidrug resistance isolates [P =0.04]. So 14MDs plasmid could determine virulence of isolates
ABSTRACT
To compare the therapeutic effect of pulsed electromagnetic field [PEMF], laser and their combination in the treatment of primary periarthritis shoulder. Forty five patients with primary periarthritis shoulder were included in this study. They were divided randomly into 3 groups according to the line of treatment [15 patients each]. Group I: treated by PEMF with intensity of 3 mT and frequency of 4 Hz for 20 minutes per session. Group II: treated by GaAiAr IR-diode laser therapy of 880 nm wave length with a dose equal to 1 joule/cm[2] for 20 minutes per session. Group III: treated by combination of PEMF and laser in the same time and same parameters of the previous 2 groups. The patients of the 3 groups were subjected to exercise program. The sessions were continued for 2 months, 3 times per week. The follow up period was 2 months during which the patients were instructed to continue their exercise program at home. In the 3 studied groups there was statistically significant improvement in all shoulder parameters [pain, tenderness, range of motion and function] after treatment and in the follow up period compared to before treatment. The comparison of improvement in all shoulder parameters after treatment and follow up revealed statistically significant difference among the 3 studied groups with best improvement was in group III followed by group II, and lastly group I. PEMF therapy, laser therapy and their combination have proved to be efficient physical modalities for treating primary periarthritis shoulder. They lead to improvement in all shoulder parameters. Also, they are safe and have long lasting effects
Subject(s)
Humans , Male , Female , Shoulder Joint , Electromagnetic Fields , Low-Level Light Therapy , Follow-Up Studies , Combined Modality Therapy , Surveys and Questionnaires , Recovery of FunctionABSTRACT
Background and Objective: it is still unclear whether Thl /Th2 cytokines are involved in the pathogenesis of hepatocellular injury of hepatitis C infection. We therefore examined serum levels of IL-10 and IL-12 in chronic liver disease patients, whereas IL-12 represents Thl cytokine and IL-10 represents Th2 cytokine
Methods: serum levels of LL-10 and IL-12 were measured in 54 patients including 30 with chronic hepatitis [CH] and 24 with liver cirrhosis [LC] in comparison with 20 normal individuals, by an enzyme-linked immunosorbent assay
Results: serum level of IL-10 was significantly higher in chronic hepatitis and LC than in controls [89.6 +/- 56.58 pg/ml ad 1 73.38 +/- 67.71 pg/ml Vs 36.35 +/- 16.04 pg/ml, respectively, P<0.001, each]. It was significantly higher in LC than in CH [p<0.001] Serum level of IL-12 was significantly higher in CH and LC than in controls [325.03 +/- 132.75 pg/ml. 349.01 +/- 204.32 pg/ml Vs 184.15 +/- 122.16, P<0.001 and P<0.01, respectively]
Conclusion: the results of the present study suggest that Th1 /Th2 type cytokines are changed in association with progression of chronic liver disease type related to HCV infection