ABSTRACT
Background: Knowledge about assisted reproductive techniques [ART] and its procedures affect the attitude of infertile people. Making decisions about the use of ART is affected by one's perception and attitude
Objective: The aim of this cross-sectional study was to determine the attitude of infertile couples toward applying ART, and to investigate its related factors
Materials and Methods: A randomized cross-sectional study was conducted on 184 infertile couples who had referred to the Research and Clinical Center of Infertility, Yazd, Iran for diagnosis and treatment in June 2014. The data was collected using a two-part questionnaire containing demographic and attitudinal statements. For data analysis, SPSS statistical software and statistical tests of mean differences [t-test], Pearson correlation and analysis of variance were used
Results: A significant relationship between spouse's attitude [p<0.01], relative's attitude [p<0.01], the applied knowledge of ART [p<0.01], and attitude of infertile couples toward applying the ART was observed; however, there was not any significant relationship between gender and socioeconomic status toward applying ART [p>0.05]
Conclusion: In conclusion, making a decision and accepting ART can be influenced by couple's attitude, their family's attitude and applied knowledge of ART
ABSTRACT
Objective:To analyse molecular detection of coliforms and shorten the time of PCR. Methods:Rapid detection of coliforms by amplification of lacZ and uidA genes in a multiplex PCR reaction was designed and performed in comparison with most probably number (MPN) method for 16 artificial and 101 field samples. The molecular method was also conducted on isolated coliforms from positive MPN samples; standard sample for verification of microbial method certificated reference material; isolated strains from certificated reference material and standard bacteria. The PCR and electrophoresis parameters were changed for reducing the operation time. Results:Results of PCR for lacZ and uidA genes were similar in all of standard, operational and artificial samples and showed the 876 bp and 147 bp bands of lacZ and uidA genes by multiplex PCR. PCR results were confirmed by MPN culture method by sensitivity 86%(95%CI:0.71-0.93). Also the total execution time, with a successful change of factors, was reduced to less than two and a half hour. Conclusions:Multiplex PCR method with shortened operation time was used for the simultaneous detection of total coliforms and Escherichia coli in distribution system of Arak city. It’s recommended to be used at least as an initial screening test, and then the positive samples could be randomly tested by MPN.