ABSTRACT
Osteoarthritis is the commonest form of arthritis and is considered to be one out of ten major causes of disability in the world. The objective of present study was to investigate the effect of treadmill exercise alone and in combination with quercetin supplementation on male rats' knee osteoarthritis. Thirty male Wistar rats [weight of 173 +/- 1 g, 8-weeks old] were randomly divided into 5 experimental groups [N=6]: intact control, MIA only, training, quercetin, training plus quercetin. The Osteoarthritis model was induced by intra-articular injection of monosodium iodoacetate [MIA]. Subjects then followed a moderate-intensity exercise program and quercetin supplementation for 28 days. Rats were killed after 28 days and histological assessment has been performed on their knee joints. One-way ANOVA [p<0.05] and post-hoc Tukey test was used for the statistical analysis. Histological assessment including 1- Depth Ratio of Lesions [p=0.001], 2- Total Degeneration Width [p=0.001] and 3- Significant Degeneration Width [p=0.001] demonstrated a beneficial influence of moderate exercise combined with quercetin supplementation group, but this influence was not superior than the moderate exercise alone group. This study shows that a moderate exercise program and quercetin supplementation, either alone or in combination, exert a beneficial influence on rats' knee osteoarthritis. But it appears that moderate exercise alone has more effectiveness
ABSTRACT
Osteoarthritis is the most common form of synovial joint diseases which affect old people all around the world and can lead to chronic pain and severe disability. The aim of present study was to investigate the therapeutic effect of swimming exercise on MIA-induced osteoarthritis in male rats' knee joint. Eighteen Wistar male rats [weight of 173 +/- 1 g, 8 weeks old] were randomly divided into 3 experimental groups: intact control, monosodium Iodoacetate [MIA] only and swimming. Intra-articular injection of MIA [50 microl] was applied on rats' right knee, similarly saline was injected in left knees. Training program used in the study was swimming with moderate intensity for 28 days. After the completion of the protocol, rats were killed and both knees of the animals were assessed histopathologically. One-way ANOVA [p<0/05] and post-hoc Tukey test were used for statistical analysis of data. Results indicated that moderate swimming training for 4 weeks significantly improved osteoarthritic symptoms of rats' knee in 3 histopathological measures of Depth Ratio of Lesions [p=0/001], Total Degeneration Width [p=0/001] and Significant Degeneration Width [P=0/001]. The findings of the present study showed that a moderate swimming training program exerts a beneficial influence on cartilage lesions induced by monosodium iodo acetate injection. Thus, the training protocol used in this study can be recommended for the treatment of osteoarthritis