ABSTRACT
Objective: OCT4B1, a novel variant of OCT4, is expressed in cancer cell lines and tis-sues. Based on our previous reports, OCT4B1 appears to have a crucial role in regulating apoptosis as well as stress response [heat shock proteins [HSPs]] pathways. The aim of the present study was to determine the effects of OCT4B1 silencing on the expression of high molecular weight HSPs in three different human tumor cell lines
Materials and Methods: In this experimental study, OCT4B1 expression was suppressed in AGS [gastric adenocarcinoma], 5637 [bladder tumor] and U-87MG [brain tumor] cell lines using RNAi strategy. Real-time polymerase chain reaction [PCR] array was employed for expression level analysis and the fold changes were calculated using RT2 Profiler PCR array data analysis software version 3.5
Results: Our data revealed up-regulation of HSPD1 [from HSP60 family] as well as HSPA14, HSPA1L, HSPA4, HSPA5 and HSPA8 [from HSP70 family] following OCT4B1 knock-down in all three cell lines. In contrast, the expression of HSP90AA1 and HSP90AB1 [from HSP90 family] as well as HSPA1B and HSPA6 [from HSP70 family] was down-regulated under similar conditions. Other stress-related genes showed varying expression pattern in the examined tumor cell lines
Conclusion: Our data suggest a direct or indirect correlation between the expression of OCT4B1 and HSP90 gene family. However, OCT4B1 expression was not strongly correlated with the expression of HSP70 and HSP60 gene families
ABSTRACT
Absent in Melanoma 2 [AIM2] is an intracellular microbial dsDNA sensor which plays an important role in production of proinflammatory cytokines through Apoptosis associated Speck-like protein containing a Caspase activation and recruitment domain [ASC] and Caspase-1. Micro-RNAs [miRNAs] play important roles in regulation of immune related genes. However, there is little information regarding the effects of miRNAs on the AIM2 and ASC expression. To determine the mRNA levels of AIM2 and ASC in Jurkat cell line following introducing miRNA-143 [MiR-143]. MiR-143, a scrambled sequence and PBS were introduced separately, to the Jurkat cell lines and the mRNA levels of AIM2 and ASC were examined in parallel with beta-actin and GAPDH [as housekeeping genes] using Real-Time PCR technique. The mRNA levels of AIM2 and ASC were significantly increased in the MiR-143 transfected Jurkat cells when compared to the scrambled sequence or PBS treated cells. MiR-143 can lead to increased expression of AIM2 and ASC mRNAs. Considering the significance of AIM2 and ASC in DNA sensing and inflammosome formation, it can be considered as a therapeutic agent for the treatment of chronic infectious diseases, especially viral infections
ABSTRACT
Chemokines and their receptors are expressed in different types of malignancies. CC chemokines MIP-1alpha [CCL3], MIP-1beta [CCL4] and RANTES [CCL5] is believed to be anti-tumor and also aid to the metastasis in tumor microenvironment. CCR2 and CCR5 are special G-protein receptors for these chemokines. Due to the important role of CCR5 chemokine receptor in tumor biology, this project is designed to examine delta 32 mutation in CCR5 gene regards breast cancer. This experimental study was performed during 2007-8 on delta healthy adults and 36 breast cancer patients by Gap-PCR. The demographic information also was collected by questionner and t-test Chi-square was used for statistical analysis of data. Our results showed that none of breast cancer patients had CCR5-delta 32 mutation while 3 [3%] cases of controls had heterozygotic form of this mutation. Our results showed that there is not any CCR5-delta 32 mutation in patients. Therefore, it appears that this mutation don't play any role in breast cancer