[Comparison three methods, PCR method, cultyre and rapid urease test to detect helicobacter pylori in the gastric biopsy tissue samples]

Background and Objectives: helicobacter pylori prevalence is high in developing countries. This microorganism is accepted as the most important agent of gastritis and as a risk factor for peptic ulcer disease and gastric adenocarcinoma. Currently many diagnostic methods exist for detecting H. pylori, however they all have limitations. The aim of this descriptive study was to evaluate diagnostic methods, such as rapid urease test, culture and polymerase chain reaction [PCR]

Materials and Methods: for identification of Helicobacter pylori 100 patients [46 women and 54 men] who were suffering from digestive complaints and referred to the endoscopy department of Hajar Hospital in Sharkord were participated in the study. Gastric biopsy samples were collected from each patient, then polymerase chain reaction, culture and rapid urease test were performed. DNA extraction was followed by PCR was used for diagnosis of ureC gene

Results: biopsy samples from100 patients were evaluated. H. pylori was positively identified by PCR in 78/100 [78%] of the patients, while positive samples were found in 52 [52%] and 48 [48%] of the patients by RUT and culture methods, respectively. In results of the culture, there was an agreement of 100% with PCR

Conclusion: three different methods for the detection of Helicobacter pylori were evaluated. Given that the PCR test has higher sensitivity and specificity to detect H.pylori comparing rapid urea as test, therefore this method could be used to detect H.pylori

[Evaluation of the frequency of methicillin-resistant staphylococs isolated from nose of nursing personnel of Hajar hospital of Shahrekord]

Methicillin-resistant Staphylococcus strains, as one of the most important etiologic agents of nosocomial infections are of particular importance due to their ability to create potential cross-resistance to all beta-lactam agents. Nasal carriers in hospital staff are supposed to be the main sources of nosocomial infection. This study was conducted aiming at determining the frequency of methicillin-resistant Staphylococcus strains isolated from nose of the personnel of Hadjar Hospital of Shahrekord. In this descriptive study, nose swabs were collected from 204 personnel of the Hajar hospital of Shahrekord. At first, the nasal swab specimens were cultured on mannitol salt agar [MSA] and TSB. Then, the Staphylococcus strains were isolated and identified using standard microbiological methods, including catalase, coagulase, DNase, and mannitol fermentation tests. In continue, agar screen method was used to determine the susceptibility of the isolated methicillin-resistant Staphylococcus strains. The results were statistically analyzed using chi-square and Fisher's exact tests. According to the results obtained, 23 of 52 [44%] Staphylococcus aureus strains and 70 strains of 152 [46%] coagulase-negative staphylococcus strains were resistant to methicillin, using agar screen method. The highest percentage of Staphylococcus aureus carriers was isolated from the staff of infectious ward and from the experienced staff [6-10 years] of a special ward. Also, there was no significant relationship between personnel's work experience in the special ward or their workplace and being a carrier. The results of this study demonstrated that the frequency of methicillin-resistant Staphylococcus strains is considerable in the personnel of Hajar Hospital. Therefore, considering the risk of its resulting epidemics in nosocomial infections among hospital's personnel, it seems necessary to detect carriers to control and prevent nosocomial infections

Clinical and bacteriologic correlates of the PapG alleles among uropathogenic escherichia coli strains isolated from cases of adult urinary tract infection

To study the distribution of papG gene in uropathogenic Escherichia coli [E.coli] strains isolated from adult urinary tract infection [UTI] and the relationship between the different classes of papG gene and patients, sex, hospitalization and their clinical forms of UTI. Laboratory study. Inpatient and outpatient settings with laboratory investigation. Genotyping of papG, the adhesion gene of E. coli P fimbriae, may predict clinical outcomes of UTI. A total of 182 urinary E .coli strains were analyzed by multiplex PCR method for detection of papG gene. Patients, sex, hospitalization and their clinical forms of UTI were also evaluated. The distribution of papG gene in uropathogenic E.coli strains and the relationship between papG gene and clinical features of the patients. Multiplex PCR method was performed for detection of papG gene in uropathogenic E.coli strains isolated from adult urinary tract infections The prevalence of pap operon in the uropathogenic isolates was 36.2%. The prevalence of papG gene classes II and III in uropathogenic isolates was 23.1% and 6.6% respectively. None of the isolates had class I genotype. PapG classes II and III were predominant in patients with pyelonephritis and cystitis respectively. There was no significant relationship between the presence of papG alleles, sex and hospitalization of the patients. PapG gene is likely to play an important role in pathogenesis of uropathogenic strains of E.coli in adult nosocomial UTIs. Detection and genotyping of this gene may contribute to improving the management of UTI

Expression and in vitro characterization of herpes simplex virus 1 [HSV-1] ORF P protein

Herpes simplex virus 1 [HSV-1] unspliced 8.3 latency associated transcript [LAT], which located in the long repeat sequences, has been shown to contain at least 16 open reading frames [ORF: A-P]. One of these ORF, ORF P, maps almost entirely antisense to HSV-1 neurovirulence gene, ICP34.5. Both ORF P and ICP34.5 are located in the long repeat and are antisense overlapping genes. Therefore, in ORF P deletion mutants, ICP34.5 is also deleted and thus, the characterization of ORF P mutants is almost impossible. An alternative way to analyse its function is to determine those cellular and viral proteins which interact with ORF P. During these experiments, firstly, the expression of full length Glutatione-S-transferase [GST]-ORF P fusion protein was optimised and then, using GST-pull down, it was shown that ORF P interacts with a viral and a few cellular proteins in vitro. Conclusively, ORF P might have some functions in HSV-1 replication cycle