ABSTRACT
Genetic diversity amongst 21 induced mutant clones tolerant to salinity along with one non-irradiated sensitive clone of banana [Musa acuminata cv. Dwarf Cavendish [AAA]] were studied using morphological and random amplified polymorphic DNA [RAPD] markers. Out of the 30 phenotypic indices screened, 23 were polymorph and two traits, leaf habit and blotches color, were differentiated by non-irradiated clone. RAPDs established 106 major amplified products using 14 primers. Out of 106 markers, eight were monomorph, and the remaining [98] were polymorph. The extent of polymorphism indicated the existence of considerable variation DNA level within induced mutant clones. Primer OPA-02 revealed banding patterns specific to salinity resistant clones. Both morphological and RAPD analyses successfully detected genetic variation within induced mutant clones, RAPD also detected variation between the irradiated and non-irradiated clones, which were morphologically indistinguishable. Results were indicative that induced mutations bear a great potential in improving banana for salinity resistant
Subject(s)
Salinity , Mutation , Genetic Variation , Random Amplified Polymorphic DNA TechniqueABSTRACT
Bread wheat, with widest adaptability range in the crop plants, has borned from crossing between tetraploid wheat T.durum and Ae. tauschii, a wild diploid wheat from Iran. The D genom of Bread wheat provides by this wild donor and it is cuse of some important agronomic trits and wide adaptability of bread wheat. Whoever, genetic variability reported within the D genome of bread wheat is much lower than Ae. tauschii populations. Therefore, genetic diversity of this species offers a great potential for wheat improvement quality and adaptability characteristics. The genetic diversity in D genome, its application methods and some of highlighted results in plant breeding were reviewed here
ABSTRACT
Little information is available regarding genetic variation in wild wheat relatives from Iran. In this study, genetic diversity of 36 populations of wild einkorn wheat, Triticum boeoticum, was studied using amplified fragment length polymorphism [AFLP] primer combinations. Seventeen AFLP primer combinations led to amplify 979 scorable fragments ranging from 50 to 500 bp and of these, 429 [44%] were polymorphic across the 36 populations. The average Dice genetic similarity between T. boeoticum populations was 0.67 [range= 0.18-0.98]. The dendrogram derived by unweighted pair group method with arithmetic mean algorithm [UPGMA] analysis revealed three main groups. PCO analysis also confirmed subgrouping obtained by cluster analysis. The measured relative genetic distances among accessions was not correlated with geographical distances of places of their origins, indicating that the populations are genetically different. The results demonstrated that AFLP technology is a suitable technique for genetic resource management in T. boeoticum populations of these studied origin sites