Expression Patterns of Host Inflammatory Cytokine Genes during Infestation with Haemaphysalis longicornis, a Zoonotic Vector, in Blood Sucking Periods

Tick saliva is critically important for continuous attachment to the host, blood feeding for days, and transmission of tick-borne pathogens. To characterize the patterns of inflammatory cytokine gene expression during its attachment and blood sucking time, peripheral blood samples of rabbits infested with Haemaphysalis longicornis ticks were collected at different intervals. Blood histamine concentration was evaluated as well as gene encoding IFN-γ, TNF-α, IL-2, IL-6, IL-4, and IL-10 were compared with non-infested rabbits. Blood histamine concentration of tick-infested rabbits during fast feeding time was significantly higher than that of non-infested rabbits. In both nymph and adult tick infested rabbits, expression of TNF-α and IFN-γ genes were decreased significantly (P < 0.05), while expression of IL-4, IL-6, and IL-10 were increased 1.3 to 7 folds in adult infested rabbits with the exception of IL-6 that was significantly (P < 0.05) decreased in nymph infested rabbits. IL-2 was not expressed in either nymph or adult infestation. H. longicornis saliva is capable of modulate host responses through a complex correlation with histamine and Th1, Th2 mediated cytokines that suppress the inflammatory responses directed toward inflammatory mediators introduced into the host during tick feeding.

Tiger bite: an unapprehended injury

This short communication describes the various modes of presentation, complications and management of tiger bite. All patients presenting to the emergency department with a history of tiger bite were included in the study. A total of 6 patients were admitted at the Department of Surgery, Khulna Medical College Hospital, Bangladesh, from 2003 to 2006. Injury involved different parts of the body; predominantly, the upper part. All patients needed emergency surgery including wound toileting, debridement and even reconstructive surgery. One patient died due to infective complications. Tiger bite is a major public health problem in the greater Sundarban area of Bangladesh. Timely hospitalization and effective management can save valuable human lives