Antimicrobial, anti-oxidant and hepatoprotective activities of Quinazolinone-4 derivatives on Paracetamol induced hepatotoxicity in Guinea pigs

In this study, two new compounds of quinazolinone-4 derivatives were synthesized and evaluated for some of their pharmacological activities that were predicted by computer program [PASS], and also testified for their yield obtained, by using some of strong polar aprotic solvents. The synthesis of new compounds in lab from 2-phenylbenzoxazinones-4 with some compound as [p-aminobenzenesulfonamides] in a medium of acid catalyst and strong polar aprotic solvents [DMSO and DMFA]. After purification and identification of the final synthesized products; known weight was dissolved in 1ml of DMSO. This solution was used to determine the antimicrobial activity, using diffusion method. Results showed that using of catalytic quantity of strong polar aprotic solvents increased the yield by 25% with DMSO and 12.5% with DMFA. A study of the in-vitro of compounds [A and B] shows no antibacterial activity. It is suggested that the activity will be shown clearly in experiments in vivo because these compounds will be metabolized into the corresponding sulfonamides. The synthesized compounds have been investigated for their toxicity and anti-oxidant activity [in vivo] by testing activity in inhibition of hepatotoxicity induced that the new compounds have low toxicity; since compound [A] gave class VI [harmless] and compound [B] gave class V [practically non toxic] according to Sidorov's classification. In the anti-oxidant study, the investigated compounds A and B showed high anti-oxidant activity, [29.08% and 48.2% respectively] higher than the compared standard. It is concluded that that the new synthesized quinazolinone-4 derivatives are preferably obtained with usage of strong polar aprotic solvents, especially DMSO. In addition, the pharmacological investigations have confirmed the prediction of PASS that testifies the high reliability of the obtained results.

Antisperm antibodies in chronic bacterial prostatitis

Antisperm antibodies are probably responsible for a number of cases of unexplained infertility. There are several factors responsible for antisperm antibodies formation, chronic prostatitis is an important one. Sixty patients suffering from chronic prostatitis and twenty normal fertile controls are enrolled into this study. Mixed antiglobulin reaction [MAR] test was used to detect antisperm antibodies in their semen while sperm immobilization test was done to detect antisperm antibodies in their serum. The results of this study showed that the duration of chronic prostatitis significantly affect fertility. There was non statistically significant difference in MAR test results in patients with chronic prostatitis as compared to normal controls. There was a statistically significant difference of sperm immobilization test results in patients as compared to normal controls. There was a significant relation between the duration of chronic prostatitis and positive MAR test sperm immobilization test results. The incidence of MAR test positive cases was higher in infertile patients than fertile cases and normal controls, but this did not reach the level of statistical significance. Sperm immobilization test results showed a statistically significant positivity in infertile patients as compared to fertile, single and normal controls. There was non statistically significant difference between sperm count or motility and positivity of either MAR test or sperm immobilization test