ABSTRACT
This study was carried out to investigate the effect of saffron [Crocus sativus], basil [Ocimum sanctum], and red cabbage[ Brassica oleracea var. capitata f. rubra] on hepatic damage in rats. Seventy two adult male albino rats of Sprague-Dawley were divided into two main groups. The first main group [n=8] was kept as a negative control group [-ve].The rest of the animals [n=64] were subcutaneously injected with a single dose of CCL4 for the induction of necrosis. After injection of CCL4, rats were divided into 8 subgroups [n=8]. The first subgroup of rats continued as a positive control group [+ve]. the other subgroup were fed on the experimental diets containing different levels of saffron, basil, red cabbage and their mixtures. At the end of the experiment a period, liver enzymes such as ALT, AST, ALP, gamma glutamile transpeptidase [GGT], lactate dehydrogenase [LDH] were determined. Total protein, albumin, total and direct billirubin were determined. As well as histopathological examinations of organs [ liver and kidney]. Results indicated that feed intake was improved in all groups except group of rats fed on experimental diet containing 10% saffron. Body weight gain and feed efficiency ratio of ll groups were decreased when compared with negative control group moreover, liver enzymes ALT, AST, ALP in addition GGT, LDH, albumin, total and direct billirubin were decreased in the treated groups compared with positive control group. This study concluded that saffron, basil and red cabbage have a protective effect against hepatic damage in rats. This study recommends the inclusion of saffron, basil and red cabbage in the meals of patients with hepatic damage for possible beneficial effect
ABSTRACT
The present study was conducted in order to examine the protective effect of lemon grass [Cymbopogon citratus] water extract [LGWE] against nephrotoxicity induced by cisplatin of male albino rats. Thirty five adult male albino rats weighing between 120-140g were randomly separated into five different groups [7rats each]. Group 1 was a normal control group [-ve], fed on basal diet. Group 2 was the positive control group [+ve] fed on basal diet for 6 weeks and then injected intraperitoneally [i.p.] with a single dose of cisplatin 5mg/kg of body weight. Groups 3,4 and 5 fed the same as group 2 and received 5,7.5 and 10% lemon grass water extract, respectively, for 6 weeks and then injected intraperitoneally [i.p.] with the same dose of cisplatin. 5 days later all rats in all groups were sacrificed and the blood was collected for biochemical and histopathological investigation. Cisplatin treatment caused significantly increase in serum malondialdehyde, uric acid, blood urea nitrogen and creatinine as well as alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase [p<0.05] in +ve control group compared to -ve control group. Rats which were fed LGWE [ groups 3, 4, and 5] showed marked reduction in the same biochemical investigations compared to +ve control group. Reduced glutathione [ GSH], serum sodium and potassium mean values were decreased in +ve control group compared to -ve control rats. Feeding LGWU in groups 3,4 and 5 showed a rise in the same biochemical parameters compated to +ve control group. 2,2-diphenyl-1-picrylhydrazyl [ DPPH], half maximal inhibitory concentration [IC50] and total phenolic content of lemon grass was assayed. Parallel to the above mentioned changes, cisplatin treatment enhances renal damage as evidenced by sharp impairment of kidney function correspondence to biochemical parameters and histopathological findings. Additionally feeding LGWE caused gradually histopathological improvement in renal tissues in groups 3,4,and 5. These results of this present study indicating that aqueous extracts of cympogon citratus has antinephrotoxic properties against cisplatin include renal oxidative damage in rates which might be ascribed to its antioxidant and free radical scavenging property. According to these above results, it is recommended to conduct further studies on the use of LGWE and possible protection of human being against nephrotoxicity