Effect of adrenaline and ATP on glycerinated skeletal muscle

Glycerinated psose muscles of rabbit were used to study the effect of adrenaline and ATP on the contractile machinery of skeletal muscle. The muscles were placed in pure glcerine for 22 days to rupture all the membranes and then a few fibres were stripped off. The tension was recorded with the help of a highly sensitive force transducer in the presence of adrenaline and ATP. The results obtained showed a 19% higher tension generated in the presence of adrenaline and this increase in tension was significant statistically. Since the muscles used were devoid of their membranes and electrical potentials, it was assumed that drug produced higher tension by acting directly on the contracticle proteins themselves