ABSTRACT
Aim: The study aimed at analyzing ESBL and AmpC positive Enterobacteriaceae in the gastrointestinal tracts of healthy community subjects and hospitalized patients and detection of blaCTX-M type gene in ESBL positive isolates. Methods: Bacteria were isolated from stool samples of the study population. Production of ESBL-type beta-lactamases was screened by double-disk synergy test as well as automated system, and AmpC enzyme production was detected by the AmpC disk test. ESBL positive isolates were subjected to detection of the blaCTX-M gene. Results: A total of 792 stool samples (50% from healthy subjects and 50% from hospitalized patients) were studied. The prevalence rates of ESBL-positive Enterobacteriaceae were 9.3% in hospitalized patients and 4.4% in healthy community subjects. Production of the AmpC enzyme was detected in
ABSTRACT
Liquid chromatography tandem mass chromatography (LC-MS/MS) is an important hyphenated technique for quantitative analysis of drugs in biological fluids. Because of high sensitivity and selectivity, LC-MS/MS has been used for pharmacokinetic studies, metabolites identification in the plasma and urine. This manuscript gives comprehensive analytical review, focusing on chromatographic separation approaches (column packing materials, column length and mobile phase) as well as different acquisition modes (SIM, MRM) for quantitative analysis of glucocorticoids and stimulants. This review is not meant to be exhaustive but rather to provide a general overview for detection and confirmation of target drugs using LC-MS/MS and thus useful in the doping analysis, toxicological studies as well as in pharmaceutical analysis.
ABSTRACT
The most common injuries to the eyes during general anaesthesia are corneal abrasions. Patients undergoing oral, maxillo-facial, dental eye and ENT surgeries are at an increased risk due to proximity of the eye to the area of surgery. We have reviewed the literature on the etiology of corneal injuries and discussed the techniques of eye protection