ABSTRACT
<p><b>OBJECTIVE</b>To investigate the genetic linkage between several cytokine and cytokine-related receptor gene loci and essential hypertension (EH) in Chinese.</p><p><b>METHODS</b>Linkage between seven genetic markers and EH in 95 Chinese nuclear families with EH (including 477 subjects) was analyzed using a technique of fluorescence-based gene scan with DNA short tandem repeat loci. These markers were selected from the chromosomal regions nearby eight cytokines and their receptor genes. The two-point non-parametric linkage analysis (NPL), maximum Lod score and transmission/disequilibrium test (TDT) with GENEHUNTER software package were used in this study.</p><p><b>RESULTS</b>Result of TDT showed significant transmission disequilibrium between D14S61 and EH (Chi square 14.29,P=0.00016) although NPL and Lod score revealed no significant linkage (Z=0.78, P>0.05 and Lod score =0.72 respectively) at this locus. No linkage between other loci typed and EH was found by the three genetic analysis methods (P>0.05 or Lod score<-1).</p><p><b>CONCLUSION</b>Alleles at D14S61 were of significant transmission disequilibrium in affected siblings. Transforming growth factor beta 3 is 0.1 cM away from D14S61, which suggests that the relationship between genes at or near this regions and EH needs to be further explored.</p>
Subject(s)
Adult , Female , Humans , Male , Middle Aged , Alleles , Blood Glucose , Metabolism , Blood Pressure , Physiology , Body Mass Index , China , Cholesterol , Blood , Cholesterol, HDL , Blood , Cholesterol, LDL , Blood , Cytokines , Genetics , DNA , Genetics , Family Health , Genetic Linkage , Hypertension , Blood , Genetics , Linkage Disequilibrium , Lod Score , Microsatellite Repeats , Receptors, Cytokine , Genetics , Triglycerides , BloodABSTRACT
<p><b>OBJECTIVE</b>To verify the linkage of the candidate regions identified in a previous study (markers D2S168, D2S151, D2S142 on chromosome 2) with hypertension in Chinese families.</p><p><b>METHODS</b>A genetic linkage study focused on chromosome 2 was performed on 240 Chinese families containing 856 patients with essential hypertension. A total of 1080 individuals were genotyped using 9 highly polymorphic microsatellite markers around the candidate regions on chromosome 2 with an average spacing of 5 cM. Non-parametric linkage (NPL), parametric linkage analysis and transmission-disequilibrium test (TDT) with the GENEHUNTER software were used to assess evidence for linkage.</p><p><b>RESULTS</b>Linkage of a region on chromosome 2 around D2S151 and D2S142 with hypertension was confirmed by different statistical methods (NPL, LOD score and TDT). However, the linkage of D2S168 could not be replicated in this extension study.</p><p><b>CONCLUSIONS</b>The data suggest that a region on chromosome 2 at or near the loci of D2S142 and D2S151 may harbor genes responsible for the development of essential hypertension in Chinese.</p>