ABSTRACT
Molecular methods are the gold standard for diagnosis of Mycoplasma, due to the difficulty of culture isolation and variability of antibody response to serologic assays. This study aimed at comparing the results of a commercially available culture system for Mycoplasma hominis [M. hominis] and Ureaplasma to the results of the detection using PCR amplification. A total of 160 endo-cervical swabs have been collected "2 swabs from each of 40 infertile females undergoing intra-cytoplasmic sperm injection "ICSI", as well as from the control group which included 40 multiparous women". All samples were subjected to isolation and identification of urogenital mycoplasmas [Bio-Rad,USA] and PCR amplification using specific primers for M. hominis and Ureaplasma urealyticum [U. urealyticum]. PCR revealed 7 cases and 2 controls were positive to M. hominis, while 12 cases and 16 controls were positive to U. urealyticum. The Duo kit revealed 9 cases and 2 controls were positive to M. hominis while 14 cases and 16 controls were positive to U. urealyticum. The sensitivity of Duo kit in relation to PCR was 89.47% and the specificity was 90.48. This work confirms the reliability of Mycoplasma Duo kit culture system for diagnosis of genitourinary colonization of the female genital system with M. hominis or U. urealyticum. Besides, higher sensitivity than the PCR was reported