Multi-drug resistant uropathogenic isolates of proteus at Assiut university hospitals

Background: proteus bacilli are more commonly associated with UTIs in those individuals with structural or functional abnormalities, especially ascending infections in patients undergoing urinary catheterization

Aim of the study: to estimate the frequency of proteus infection in patients with I urinary tract infections, detection of sensitivity profile to antibiotics and detection of ESBL

Material and methods: one hundred and fifty five patients with symptoms of urinary tract infection were recruited and urine sample from each patient was collected as well as demographic data and associated I risk factors were recorded. Urine culture, antimicrobial susceptibility test, screening and confirmatory tests for detection of ESBL were done

Results and conclusion: the mean age +/- SD was 45 +/- 17 years, 139 were males and 16 were females. Sixty eight patients were catheterized and 87 patients were non-catheterized. E coli was the highest bacterial organism [35%] detected by urine culture among catheterized patients, while Kpneumoniae was the highest among non-catheterized patients. There was a significantly higher percent of proteus among non-catheterized patients than catheterized patients. Proteus alone or proteus with other bacterial infection was detected in 14.8% in catheterized versus 6.1% in non-catheterized patients. Only 13 cases out of 155 cases [8.4%] had positive culture for proteus organism. All are males and most of them were catheterized and had benign enlarged prostate [8 cases; 61%]. Out of 13 Proteus isolates, nine Proteus isolates showed reduced susceptibility to the Ceftazidime, Cefotaxime, Ceftriaxone, and Cefuroxime and gave the same zone diameter range as adapted by CLSI [2014] document M100 S24 and nine considered potential ESBL producers

Antibiotic susceptibility patterns of propionibacterium acnes isolated from acne vulgaris in Assiut university hospitals, Egypt

Background: Antibiotics are frequently used to treat acne patients either as bactericidal or anti-inflammatory agents. However, with the increased use of antibiotics, resistant strains of Propionibacterium acnes began to emerge and have been associated with a poor treatment outcome

Objectives: Detection of staphylococcal and Propionibacterium acnes strains in cases of acne vulgaris in Assiut university hospitals, Egypt and antibiotic susceptibility patterns of Propionibacterium acnes isolates

Methodology: Microbiological samples were obtained from one hundred patients with inflammatory acne lesions. Samples were cultured on blood agar and mannitol salt agar media under aerobic conditions at 37[degree]c for isolation of staphylococcal strains, and on blood agar under anaerobic conditions at 37[degree]c for 3 to 7 days for isolation of Propionibacterium acnes. Bacteria were identified by colonial morphology, standard biochemical tests, and API 20A test for identification of Propionibacterium acnes isolates. Antibiotic sensitivity testing of Propionibacterium acnes strains was done against clindamycin, erythromycin, doxycyclin, trimethoprime/sulfamethxazole, tetracycline and levofloxacin

Results: Staphylocoocal strains were detected in 55% of acne cases, while Propionibacterium acnes were detected in 35% of cases. Most Propionibacterium acnes isolates were sensitive to levofloxacin [80%], followed by doxycycline [51.4%], tetracycline, trimethoprime/ sulfamethaxazole [20.0% for each] while showed highest resistance rates to clindamycin [85.7%] and erythromycin [82.9%]

Conclusion: Levofloxacin was the most effective antibiotic for Propionibacterium acnes followed by doxycycline, while Erythromycin and clindamycin were the least effective antibiotics for Propionibacterium acnes

Molecular identification and antifungal susceptibility of pathogenic fungi isolated at Assiut university hospitals

The incidence of invasive opportunistic mycoses [10Ms] has increased because of the expanding population of immunosuppressed patients raising the demand for identification of local epidemiologic trends, the degree of virulence and antifungal susceptibility pattern of fungal pathogens. Identification of the local epidemiology of l0Ms at Assiut University hospitals showed that there is a marked shift to Candida non albicans [can], rare yeasts that cause Candida like infections, zygomycetes, hyaline moulds and a wide variety of dematiaceous fungi. In vitro susceptibility testing pattern of isolated fungal pathogens against 8 antifungal agents; Amphotericin B [AP], Nystatin [NS], l;luconalole [FU], Ketoconazole [KT], Clotrimazole [CC], Voriconazole [VOR], Itraconazole [IT] andv Terbinafinc showed that these emerging pathogens are resistant to conventional antifungals, in addition, they pose a high enzymatic and toxigenic ability making them more virulent and infections caused by them more aggressive and very difficult to manage