1.
Hamdard Medicus. 2010; 53 (4): 110-113
in English
| IMEMR
| ID: emr-131132
ABSTRACT
A simple spectrophotometric method based on the reaction with Bromocresol Green [BCG] has been developed for determination of total alkaloids in medicinal plants. A yellow complex forms and is easily extractable by chloroform at pH 4.7. The absorbance of the complex obeys Bear's law over the concentration range of 4-13 micro g atropine per mL of choloroform. This procedure can be carried out in the presence of other compounds without interference