Padronizaçäo do uso de areia do mar na remoçäo de leucócitos de sangue bovino / Standardisation of the use of limestone particles to remove leukocytes from bovine blood

The objective of this paper was to evaluate different volumes of limestone particles in the removal of leukocytes from bovine blood. Samples of red blood cell suspensions were tested before and after filtration for presence of leukocytes using Acridine Orange and counting in Neubauer chamber. The proportion of red blood cells lost in each filtration was also calculated. It was concluded that filtration through a volume of 10 cm3 of limestome particles per ml of suspension allowed removal of leukocytes with little losses of red blood cells in the column. The technique may be useful in purification of hemoparasites in which the removal of leukocytes is essential