ABSTRACT
Cardiovascular disease is one of the leading causes of death worldwide, and evidence indicates a correlation between the inflammatory process and cardiac dysfunction. Selective inhibitors of cyclooxygenase-2 (COX-2) enzyme are not recommended for long-term use because of potentially severe side effects to the heart. Considering this and the frequent prescribing of commercial celecoxib, the present study analyzed cellular and molecular effects of 1 and 10 µM celecoxib in a cell culture model. After a 24-h incubation, celecoxib reduced cell viability in a dose-dependent manner as also demonstrated in MTT assays. Furthermore, reverse transcription-polymerase chain reaction analysis showed that the drug modulated the expression level of genes related to death pathways, and Western blot analyses demonstrated a modulatory effect of the drug on COX-2 protein levels in cardiac cells. In addition, the results demonstrated a downregulation of prostaglandin E2 production by the cardiac cells incubated with celecoxib, in a dose-specific manner. These results are consistent with the decrease in cell viability and the presence of necrotic processes shown by Fourier transform infrared analysis, suggesting a direct correlation of prostanoids in cellular homeostasis and survival.
Subject(s)
Animals , Rats , Cell Proliferation/drug effects , Cell Survival/drug effects , /pharmacology , Gene Expression Regulation/drug effects , Myoblasts, Cardiac/drug effects , Pyrazoles/pharmacology , Sulfonamides/pharmacology , Blotting, Western , Cell Line , Cell Proliferation/genetics , Cell Survival/genetics , Dose-Response Relationship, Drug , Gene Expression Regulation/genetics , Reverse Transcriptase Polymerase Chain Reaction , RNA, Messenger/drug effects , RNA, Messenger/genetics , Spectroscopy, Near-Infrared , Time FactorsABSTRACT
Por meio do cotrimoxazol, foram tratados 30 individuos, representados por criancas e adultos, com pediculose da cabeca. A conduta usada correspondeu a administracao de um comprimido com 400 mg de sulfametoxazol e 80 mg de trimetoprim cada 12 horas, durante tres dias, com repeticao de igual serie medicamentosa depois de intervalo com dez dias de duracao. Ocorreram 28 (93,3%) curas, demonstrando a eficacia do novo metodo curativo referente a infestacao pelo Pediculus humanus humanus. Essas verificacoes, alem de consubstanciarem opcao sob o ponto de vista terapeutico, criam a necessidade de esclarecer o mecanismo de acao do medicamento em apreco em face a processo motivado por inseto e, tambem, estabelecem a conveniencia de procurar saber se identica atividade sucede no que concerne a outras ectoparasitoses