ABSTRACT
Background: Hesa-A is a natural compound with anticancer properties. The exact mechanism of its action in esophageal cancer is not clear, yet. The aim of this study was to evaluate the cell toxicity effect of Hesa-A on the esophageal carcinoma cell lines, KYSE-30, and cell cycle genes expression
Methods: In this study, we tested cell toxicity with MTT [3-[4,5-Dirnethylthiazol-2-yl]-2,5-Diphenyltetrazolium Bromide] assay and flow cytometry to evaluate the cell cycle arrest
Real time polymerase chain reaction was used to assess the expression of P53, PI6, P21, cyclin Dl, and cyclin Bl genes
Results: Our results showed that Hesa-A is effective in the expression of cell cycling check point proteins. Hesa-A induced an arrest in G2 phase of esophageal cell cycle. The levels of P53 [>13 times], P21 [>21 times], PI6, cyclin Bl, and cyclin Dl genes were increased 48 hours after Hesa-A treatment
Conclusion: P21 and P16 expression were the potential mechanisms for G2 arrest of KYSE-30 esophageal cancer cell line by Hesa-A