ABSTRACT
We report the in vitro inhibitory effect of very low concentrations of aluminum salts (IC50 = 4.1 x 10-12M) on bovine brain acetylcholinesterase (AChE). The enzymatic assays were performed using acetylcholine bromide in a buffered pH 7.4 solution at 37§C. The relevant enzyme interacting species is the Al3+ ion, whose concentrations were fixed at pM levels by a citrate metal ion buffer system. The IC50 demonstrates that Al3+ is a potent inhibitor of AChE
Subject(s)
Animals , Cattle , Acetylcholinesterase/metabolism , Aluminum/pharmacology , Cerebrum/enzymology , In Vitro Techniques , Aluminum/blood , Aluminum/toxicity , Cholinesterase Inhibitors/pharmacology , Citrates/pharmacologyABSTRACT
Padronizaram-se as condicoes cromatograficas (C.C.D.) para caracterizacao da droga e do extrato fluido de Pothomorphe umbellata (L) MIq utilizando como fase movel benzeno ou as misturas de n-hexano e acetato de etila, etanol e acetato de etila.A visualizacao das manchas foi feita com luz UV 366 nm, antes da revelacao e, com luz natural apos revelacao com anisaldeido sulfurico ou cloreto ferrico a 4%. O nerolidilcatecol foi encontrado somente nas raizes. As asaronas nao foram detectadas nem nos extratos fluidos nem nos oleos esseciais da especie