ABSTRACT
Resumen La infección por el virus de la hepatitis C (VHC) se distribuye en todo el mundo, frecuentemente se convierte en hepatitis crónica, cirrosis y hepatocarcinoma. El genoma del VHC es una molécula de ARN monocatenario, de polaridad positiva, de aproximadamente 9.6 kb de longitud. Esta revisión resume el conocimiento actual y los avances recientes en la investigación de la biología molecular del VHC.
Abstract Infection with hepatitis C virus (HCV), which is distributed worldwide, often becomes in chronic hepatitis, cirrhosis and hepatocellular carcinoma. The HCV genome is a single-stranded RNA molecule of positive polarity approximately 9.6 kb in length. This review summarizes the current knowledge of recent advances in the investigation of the molecular biology of HCV.
ABSTRACT
In the present work, immunogenicity of recombinant in vitro assembled hepatitis C virus core particles, HCcAg.120-VLPs, either alone or in combination with different adjuvants was evaluated in BALB/c mice. HCcAg.120-VLPs induced high titers of anti-HCcAg.120 antibodies and virus-specific cellular immune responses. Particularly, HCcAg.120-VLPs induced specific delayed type hypersensitivity, and generated a predominant T helper 1 cytokine pro file in immunized mice. In addition, HCcAg.120-VLPs prime splenocytes proliferate in vitro against different HCcAg.120-specific peptides, depending on either the immunization route or the adjuvant used. Remarkably, immunization with HCcAg.120-VLPs/Montanide ISA888 formulation resulted in a significant control of vaccinia virus titer in mice after challenge with a recombinant vaccinia virus expressing HCV core protein, vvCore. Animals immunized with this formulation had a marked increase in the number of IFN-γ producing spleen cells, after stimulation with P815 cells infected with vvCore. These results suggest the use of recombinant HCV core particles as components of therapeutic or preventive vaccine candidates against HCV.
Subject(s)
Animals , Female , Humans , Mice , Hepacivirus/immunology , Hepatitis C/immunology , Interferon-gamma/biosynthesis , /biosynthesis , Peptide Fragments/immunology , Spleen/immunology , Viral Core Proteins/immunology , Adjuvants, Immunologic/administration & dosage , Hepatitis C/prevention & control , Mice, Inbred BALB C , Peptide Fragments/administration & dosage , Spleen/cytology , /immunology , Viral Core Proteins/administration & dosageABSTRACT
Por medio de la reacción en cadena de la polimerasa (RPC), se obtuvo una sonda para el gen que codifica la subunidad B de la toxina colérica (CTxB) que portaba una cepa de referencia de Vibrio cholerae 01. La comprobación del producto amplificado se realizó por la técnica de hibridación en colonias. El producto amplificado hibridó con el gen que codifica para la subunidad B de la toxina colérica aislada de Perú y Ecuador, representante de la presente epidemia en América Latina y no lo hizo con las cepas filogenéticamente relacionadas.
By means of the polymerase chain reaction (PCR) it was obtained a probe for the gen that codifies the subunit B of cholerae toxin (CTxB), which carried a Vibrio cholerae 01 reference strain. The checking of the amplified product was performed by using the hybridization techniques in colonies. This product hybridized with the gen that codifies for the subunit B of cholerae toxin isolated from Peru and Ecuador, representing the present epidemics in Latin America, but it did not so with the phylogenetically related strains.