ABSTRACT
RESUMO Tivemos como objetivo avaliar o efeito da infusão de Cunila microcephala Benth sobre a atividade da enzima acetilcolinesterase (AChE) e marcadores de estresse oxidativo em eritrócitos de agricultores. Foram utilizadas amostras provenientes de 16 trabalhadores rurais expostos a pesticidas agrícolas pelo período mínimo de 5 anos e um grupo controle constituído de 16 indivíduos não expostos a agrotóxicos. As hemácias dos agricultores e o grupo A foram expostos “in vitro” à solução salina (NaCl 0,9%). Os demais grupos foram expostos à infusão de poejo nas concentrações de 0; 5; 10; 25 e 50 g/L (Grupos B; C; D e E, respectivamente). Em seguida, foram realizadas as determinações da atividade da AChE e dos níveis de substâncias reativas ao ácido tiobarbitúrico (TBARS), proteínas carboniladas (PCs) e glutationa reduzida (GSH). Os resultados mostram que a infusão de poejo 50g/L, aumenta a atividade da enzima AChE e os níveis de GSH. Contudo, os níveis de TBARS e PCs diminuíram após o tratamento com a infusão de poejo 25 e 50 g/L. A infusão de poejo, na concentração de 50 g/L, é capaz de reverter, “in vitro” a inibição da atividade da AChE que ocorre pela exposição a pesticidas, e ainda demonstra um importante potencial antioxidante, tendo em vista que diminuiu danos lipídicos e proteicos e ainda, estimulou a produção do principal antioxidante não enzimático endógeno.
ABSTRACT Evaluating the effect of infusion Cunila microcephala Benth on acetylcholinesterase activity (AChE) enzyme and on biomarkers of oxidative stress in farmers erythrocytes. We used samples from 16 rural workers exposed to pesticides for a minimum of five years, and a control group composed of 16 individuals not exposed to pesticides. The erythrocytes of farmers and from group A were exposed “in vitro” the saline solution (NaCl 0,9%). The other groups were exposed to the infusion of “poejo” at concentrations of 0; 5; 10; 25 and 50 g/L (Groups B, C, D and E, respectively). Then, it was realized the analitical determinations of AChE activity and TBARS, PCs and GSH levels. The results showed that “poejo” infusion 50g/L, increased the AChE activity and GSH levels. However, the TBARS e PCs levels decreased after the treatment with “poejo” infusion 25 e 50 g/L. The “poejo” infusion 50 g/L is able to revert “in vitro” the inhibition of AChE activity that occurs by exposure to pesticides and also demonstrates an important antioxidant potential, given that decreased lipid and protein damage and also it stimulated the production of the main non-enzymatic antioxidant endogenous.
Subject(s)
Humans , Acetylcholinesterase/pharmacology , In Vitro Techniques/instrumentation , Biomarkers/analysis , Oxidative Stress , Lamiaceae/classification , Pesticides , Erythrocytes/classification , Farmers/classificationABSTRACT
Aims: To study the diversity of flowering time locus RM248 in some traditional rice cultivars in Sri Lanka and compare it with days to flowering of studied rice cultivars. Study Design: A field experiment was carried out to evaluate the days to flowering of the studied traditional rice cultivars. Rice plants were transplanted according to randomized complete block design with four replications and 20 plants per plot with 15 cm X 20 cm plant spacing. Diversity of flowering time locus RM 248 was analyzed by performing PCR using SSR marker RM 248 with genomic DNA of different eighty-nine rice genotypes. Gel electrophoresis was carried out in vertical gel electrophoresis unit for three hours. Gel plates were Silver stained and banding pattern was scored. Place and Duration of Study: Field data were collected in Sri Lanka. The experiment was carried out during 2011/2012 Maha season and 2012 Yala season at the Faculty of Agriculture, University of Ruhuna, Sri Lanka. In Sri Lanka Yala is referred as dry season while Maha is referred as wet season. Laboratory data were collected at the Laboratory of Genetics, Graduate school of Science and Technology, Kobe University, Japan from October 2012 – December 2012. Methodology: Days-to-flowering of traditional rice cultivars was studied in a field experiment and diversity of flowering time locus, RM 248 was studied by PCR amplification of SSR marker RM 248. SSR marker was amplified using the Gene Amp PCR System 9700 thermocycler. Amplified products were fractionated by electrophoresis through 6% denaturing Polyacrylamide gels and stained using Silver sequence DNA sequencing system staining regents (Promega, USA). Results: Silver stained Polyacrylamide gel showed six different allele types in traditional rice accessions in RM 248 locus. Conclusion: There is a great diversity in the RM248 locus among the traditional rice cultivars in Sri Lanka.