Investigation of polymorphisms in Plasmodium falciparum hrp2, hrp3, aldolase and pldh genes and their impact on the performance of malaria rapid diagnostic tests in Papua New Guinea

@#The World Health Organization (WHO) recommends that parasitological confirmation of clinical malaria diagnosis be performed before antimalarial treatment is administered. Malaria rapid diagnostic tests (RDTs) represent a valuable tool for prompt and efficient diagnosis of malaria in settings where microscopic diagnosis is unavailable or unreliable. Concerns remain, however, that Plasmodium falciparum polymorphisms in the genes coding the antigens detected by RDT could impact on RDT performance. Using field isolates of Plasmodium falciparum, we aimed to characterize genetic variability in histidine-rich proteins 2 and 3 (PfHRP-2 and PfHRP-3), aldolase (ALD) and Plasmodium lactate dehydrogenase (pLDH) genes and to evaluate their impact on the performance of RDT. Pfhrp-2, Pfhrp-3, aldolase and pldh were amplified using polymerase chain reaction (PCR) and sequenced. Genetic variation was observed in pfhrp-2 and pfhrp-3 genes while aldolase and pldh showed high levels of conservation. These findings suggest that RDTs based on pLDH and ALD are reliable in the study settings where there is intense diversity or polymorphisms of histidine-rich protein (HRP). Nevertheless, there is no evidence from this study to suggest that RDTs based on the detection of PfHRP-2 and PfHRP-3 have lower sensitivity in Papua New Guinea (PNG). The results observed in this study will be used to inform the PNG National Department of Health on the continued usage of pLDH/ HRP-2 RDT for malaria diagnosis in PNG.

Causes of neonatal admissions and in-hospital mortality at Modilon Hospital, Madang Province: a 5-year retrospective study

@#Approximately half of all childhood mortality in Papua New Guinea (PNG) occurs in the neonatal period – the first 28 days of life. In this 5-year retrospective study, causes of admissions and in-hospital mortality among 2426 neonates admitted to Modilon Hospital’s Special Care Nursery in Madang Province were investigated. The neonatal case fatality rate was 15% (370/2426; absolute range between years 12-22%). Neonatal sepsis/infection (1017, 42%), prematurity (821, 34%) and birth asphyxia/meconium aspiration (396, 16%) were the leading causes of admissions (92% of total neonatal admissions) and deaths (96%). Many deaths were potentially avoidable but were often complicated by multiple contributing factors. To reduce neonatal mortality in PNG, health professionals, the government, policy makers and communities must appreciate that improving new born survival and other neonatal outcomes are a responsibility for all. ].

Trends in maternal and perinatal mortality in a provincial hospital in Papua New Guinea: A 6-year review

@#In recent years, there have been increased efforts to reduce the high maternal mortality ratio (MMR) in Papua New Guinea. This retrospective study conducted at Modilon Hospital in Papua New Guinea documented maternal and perinatal mortality over the 6 years from 2009 to 2014. In-hospital maternal mortality, though still high, significantly declined by over 50% from 24/2598 (924 per 100,000) in 2009 to 12/3217 (373 per 100,000) in 2014 (p <0.001) while stillbirth rates and early neonatal death rates remained unchanged. There is a need for an approach with interventions aimed at reducing both maternal and perinatal mortality. While monitoring and auditing of maternal deaths should be possible throughout the entire country, in settings where there is limited capacity to monitor population-based perinatal and neonatal mortality, an emphasis on improved data quality as part of hospital-and health centre-based surveillance can provide important information.