ABSTRACT
Aim: Detection of protein expression changes in human cystic echinococcosis sera by 2D gel electrophoresis
Background: Diagnosis and successful treatment of cystic echinococcosis [CE] is a major challenge, up to now. Identification of related expressed proteins using proteomics tools and bioinformatics analysis of patientsf sera have not been investigated, so far
methods: Sera from eight confirmed CE patients and three healthy controls were collected, tested by 2-DE for total protein separation of serum and analyzed using proteomics and bioinformatics methods. The gels were stained by Coomassie blue followed by scan imaging of the gels. The protein spots in each gel were analyzed using progenesis same spots software. Proteins names were obtained from TagIdent server
Results: A total of 263 protein spots with different expression were detected in both normal and diseased samples. Comparison between diseased and normal gels showed the expression of 45 up-regulated protein spots with fold.2 in diseased gel of which 10 were new proteins with statistical difference by normal gel [p-value<0.05]. On the other hand, the expression of 50 down-regulated protein spots were observed of which 11 proteins have been suppressed. Clustering of all detected sera proteins [263] using correlation analysis, divided the proteins into 2 clusters based on up-regulated and down-regulated expression of proteins. Clustering results were approved by principal component analysis [PCA]
Conclusion: Significant protein expression changes in human CE sera which is demonstrable by application of proteomics and bioinformatics analysis makes it an impressing tool for diagnosis of CE patients
ABSTRACT
Introduction: During the last 3 decades, human is exposed to extremely low frequency electromagnetic fields [ELF-EMF] emitted by power lines and electronic devices. It is now well accepted that ELF-EMF are able to produce a variety of biological effects, although the molecular mechanism is unclear and controversial. Investigation of different intensities effects of 50 Hz ELF-EMF on cell morphology and protein expression is the aim of this study
Methods: SH-SY5Y human neuroblastoma cell line was exposed to 0.5 and 1 mT 50 Hz [ELF-EMF] for 3 hours. Proteomics techniques were used to determine the effects of these fields on protein expression. Bioinformatic and statistical analysis of proteomes were performed using Progensis SameSpots software
Results: Our results showed that exposure to ELF-EMF changes cell morphology and induces a dose-dependent decrease in the proliferation rate of the cells. The proteomic studies and bioinformatic analysis indicate that exposure to 50 Hz ELF-EMF leads to alteration of cell protein expression in both dose-dependent and intensity dependent manner, but the later is more pronounced
Conclusion: Our data suggests that increased intensity of ELF-EMF may be associated with more alteration in cell protein expression, as well as effect on cell morphology and proliferation
ABSTRACT
Extremely low frequency electromagnetic fields [ELF-EMF] have been common in daily life all over the world. They have produced by power lines and electrical appliances, but higher levels of them have raised a lot of concerns about their carcinogenesis. Both epidemiological and laboratory studies have suggested that EMFs might increase cancer incidence, including acute childhood leukemia, brain and breast cancer. In the present study, SH-SY5Y human neuroblastoma cell line has exposed to 2mT, 50 Hz magnetic field for 3 h. Next, effect of this exposure on protein expression including over-expression or under-expression has assessed by proteomics. Bioinformatics and statistical analysis using progenesis same spot software on the obtained 2D electrophoresis has shown that expression of 189 proteins in exposed group has changed relative to control. Besides, PCA analysis has verified results of clustering, and has shown that protein data has clustered according to experimental conditions. The results of this study have shown that ELF-EMF changes cell morphology via altering protein expression, but more profound studies have needed to determine the kind of proteins altered
Subject(s)
Humans , Neuroblastoma , Cell Line , ProteomicsABSTRACT
In this study, we report the effect of the essential oil of Rosa Damascena on human colon cancer cell line [SW742] and human fibroblast cells. Colon cancer is the second most common fatal malignancy. Owing to the existence of many side effects and problems related to common treatments such as surgery, chemotherapy and radiotherapy, alternative treatments are being investigated. Some herbal medicines have shown promising results against different types of cancers. Herbal medicines used have included the use naturally occurring essential oils. The essential oil of Rosa Damascena was obtained by distillation and its effect on SW742 cell-line and fibroblast cells were investigated with cell culture. The cells were cultured and different volumes of essential oil were induced to the cells. After 48h incubation, cell survival was measured and using statistical analysis, the findings were evaluated and reported. This study showed that soluble part of Rosa Damascena oil increases cell proliferation in high volumes and the non-soluble component decreases cell proliferation. The effects of essential oils, such as Rosa Damascena, on cell proliferation require more thorough investigation
Subject(s)
Humans , Oils, Volatile , Colonic Neoplasms , Cell Line , FibroblastsABSTRACT
The use of herbal medicine has a long history. In order of unveiling of the side effects of chemical drugs, human tried to use the natural resources to supply drugs. In this study, antimicrobial effect of Scrophularia striata aquatic extract on Staphylococcus aureus and Escherichia coli were studied and compared with the effects of tetracycline. In this basic- applied, the aqueous extract was prepared from the sterile and unsterile leaves and fruits of Scrophularia striata. Staphylococcus aureus and Escherichia coli after colony counting and determining dilution were cultured on known concentrations of the extract containing mediums and their effects on bacterial growth was assessed after 24 h by measurement of turbidity. Eighty percent of bacterial growth was inhibited by the extract, compared with 100% of control. The inhibitory effects of low concentrations of the extract were more than tetracycline. This study showed that the aquatic extract of Scrophularia striata could be a good candidate as an alternative or supplement to antibiotics to treat bacterial infections
ABSTRACT
In this study the anticancer activity of Lavender aqueous extract against MKN45 cell line was evaluated. Plant-based drugs are regarded as promising therapies. Lavender is a plant that has been cultivated from ancient times. An aqueous extract of Lavender has shown therapeutic effects on the nervous system in the high doses based on in-vivo studies. Gastric cancer is one of the frequent cancers in Iranian population. We therefore assessed the effect of Lavender upon a gastric cancer cell line. The MKN45 cancer cell line was selected for treatment with aqueous extract of Lavender Survival of MKN45 cell line was studied in the presence of various concentrations of Lavender extract by MTT assay method. Morphological studies were performed via microscopic analyses. Flow cytometry and proteomics techniques were applied to determining pharmaceutical mechanism of lavender cytotoxic effects. The survival and morphological studies revealed anticancer characteristics of extract. Flow cytometry findings indicate that Lavender extract had a cytotoxic effect upon the cell line. Proteomics analysis identified a significant spots showed changes in protein expression levels by informatics analysis. Of the proteins, expression of three cancer biomarkers, Annexin1, Anolase1 and HSP70 were suppressed by extract. This study suggest that Lavender extract is cytotoxic and alter protein expression in a gastric cancer cell line