ABSTRACT
The aim of this study was to evaluate the anti-oxidant and anti-choline esterase activity of the stem, barks and leaves of the plant V. cinerea. The stems, barks and the leaves of the plant V.Cineria was sun dried and extracted using methanol. The anti-oxidant activity of the crude methanolic extract was measured by the DPPH free radical scavenging activity. The crude methanolic extract showed significant anti-oxidant activity by the DPPH free radical scavenging method. Evaluation of ChEs enzyme activity of the crude methanolic extract was done by Ellman method. The methanolic extract of V. cinerea leaves exerted significant AChE and BChE inhibitory effects.
ABSTRACT
The antibacterial activities of n-hexane, chloroform, ethyl acetate fractions of methanolic extracts of the whole plants Paederia foetida (family Rubiaceae) were screened against various pathogenic bacteria such as Bacillus cereus, Bacillus megaterium, Bacillus subtilis, Staphylococcus aureus, Sarcina lutea, Escherichia coli, Pseudomonas aeruginosa, Salmonella paratyphi , Salmonella typhi, Shigella boydii, Shigella dysenteriae, Vibrio mimicus, Vibrio parahemolyticus, Candida albicans, Aspergillus niger, Sacharomyces cerevacae by ‘disc diffusion method’. The methanol extract of the whole plants possesses no antimicrobial activity but the ethyl acetate, chloroform and n-hexane fractions exhibited moderate to less activity against some organisms tested compared with the standard antibiotic Kanamycin. Brine shrimp lethality bio-assay was done using brine shrimp Nauplii and dimethyl sulfoxide as a solvent for the methanol plant extracts of Paederia foetida. The LC50 value of methanol extract of the plant indicated that the cytotoxicity was very significant.